The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml. Protein A affinity purification required prior to immunofluorescence application.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.