製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to NFIX
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-441 of Human NFIX

  • ポジティブ・コントロール
    • HeLa cells, Transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    ICC/IF: Use at a concentration of 10 µg/ml. Protein A affinity purification required prior to immunofluorescence application.
    WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).
    Detection Pair


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
    • 配列類似性
      Belongs to the CTF/NF-I family.
      Contains 1 CTF/NF-I DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CCAAT box binding transcription factor antibody
      • CCAAT-box-binding transcription factor antibody
      • CTF antibody
      • NF-I/X antibody
      • NF1-X antibody
      • NF1A antibody
      • NF1X antibody
      • NFI X antibody
      • NFI-X antibody
      • NFI/X antibody
      • NFIX antibody
      • NFIX_HUMAN antibody
      • Nuclear factor 1 X type antibody
      • Nuclear factor 1 X-type antibody
      • Nuclear factor 1/X antibody
      • Nuclear factor I/X antibody
      • TGGCA binding protein antibody
      • TGGCA-binding protein antibody
      see all

    画像

    • Immunofluorescence of purified ab67169 on HeLa cells. Antibody concentration 10 µg/ml.
    • All lanes : Anti-NFIX antibody (ab67169) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : non Transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 55 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab67169 has not yet been referenced specifically in any publications.

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