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Recombinant protein fragment containing a sequence corresponding to a region within amino acids 165 and 330 of Human NFIX.
Our Abpromise guarantee covers the use of ab101341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||1/1000. PubMed: 23776487|
|WB||1/500 - 1/3000. Predicted molecular weight: 55 kDa.|
Immunohistochemistry (Free Floating) analysis of adult mouse (wild type and NFix+/-) coronal brain tissue. Sections were blocked for 2 hours with serum before incubation overnight with ab101341 (1/1000 in blocking buffer). Sections were then incubated with a biotin-conjugated goat anti-rabbit IgG (1/1,000) secondary antibody for 1 hour. The reaction was visualised by incubating the sections in avidin-biotin complex for 1 hour, followed by a nickel-DAB solution, and was terminated by washing multiple times in phosphate buffered saline when a dark precipitate had formed. Sections were then mounted onto slides, dehydrated and imaged.
In adult wild-type mice (G), NFIX was most strongly expressed by cells in the SGZ of the dentate gyrus (arrowheads in G’). The same expression pattern was observed in Nfix+/− mice, although the level of NFIX expression was much lower than in wild-type mice at all ages. G’ and H’ are magnified views of the boxed regions in G and H respectively.