製品の概要

  • 製品名Anti-NFIL3 antibody
    NFIL3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFIL3
  • 特異性This antibody detects endogenous levels of total NFIL3 protein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A synthesized peptide derived from the internal region of human NFIL3

  • ポジティブ・コントロール
    • Extracts from 293 cells and K562 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.
  • 組織特異性Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
  • 配列類似性Belongs to the bZIP family. NFIL3 subfamily.
    Contains 1 bZIP domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E4 promoter binding protein 4 antibody
    • E4 promoter-binding protein 4 antibody
    • E4BP 4 antibody
    • E4BP4 antibody
    • E4BP4 protein antibody
    • IL3BP 1 antibody
    • IL3BP1 antibody
    • Interleukin 3 binding protein 1 antibody
    • Interleukin 3 promoter transcriptional activator antibody
    • Interleukin-3 promoter transcriptional activator antibody
    • Interleukin-3-binding protein 1 antibody
    • NFIL 3 antibody
    • NFIL 3A antibody
    • NFIL3 antibody
    • NFIL3_HUMAN antibody
    • NFIL3A antibody
    • Nuclear factor interleukin 3 regulated antibody
    • Nuclear factor interleukin-3-regulated protein antibody
    • Transcriptional activator NF IL3A antibody
    • Transcriptional activator NF-IL3A antibody
    see all

Anti-NFIL3 antibody 画像

  • All lanes : Anti-NFIL3 antibody (ab66004) at 1/500 dilution

    Lane 1 : Extracts from 293 cells at 5-30ug
    Lane 2 : Extracts from K562 cells at 5-30ug
    Lane 3 : Extracts from K562 cells at 5-30ug with immunizing peptide at 5-10ug


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-NFIL3 antibody (ab66004) at 1 µg/ml

    Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate (ab76827)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa


    Exposure time : 2 minutes

Anti-NFIL3 antibody (ab66004) 使用論文

ab66004 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"