製品の概要

  • 製品名Anti-NFIB / NF1B2 antibody
    NFIB / NF1B2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFIB / NF1B2
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human NFIB/ NF1B2.

    (Peptide available as ab95051.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa Whole Cell Lysate. IHC-P: human normal breast FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 配列類似性Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    • TRANSCRIPTION FACTOR NFIB antibody
    see all

Anti-NFIB / NF1B2 antibody 画像

  • Anti-NFIB / NF1B2 antibody (ab80835) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of NF1B/NF1B2 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80835, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NFIB / NF1B2 antibody (ab80835) 使用論文

ab80835 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"