製品の概要

  • 製品名Anti-NFIB / NF1B2 antibody
    NFIB / NF1B2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFIB / NF1B2
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Hamster
  • 免疫原

    Synthetic peptide:

    YVPSYDPSSPQTSQ

    , corresponding to amino acids 402-415 of Human NF1B2

  • ポジティブ・コントロール
    • MG-63 nuclear extract.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide and 0.01% Thiomerosal
    Constituents: PBS containing 0.2% Gelatin
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). A doublet may be seen in some cell lines. For optimal results, primary antibody incubations should be performed at room temperature (see recommended protocol). The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background.

ターゲット情報

  • 機能Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • 配列類似性Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • HMGIC/NFIB antibody
    • NF-I/B antibody
    • NF1-B antibody
    • NF1B antibody
    • NF1B2 antibody
    • NFI-B antibody
    • NFI-RED antibody
    • Nfib antibody
    • NFIB_HUMAN antibody
    • NFIB2 antibody
    • NFIB3 antibody
    • Nuclear factor 1 B-type antibody
    • Nuclear factor 1/B antibody
    • Nuclear Factor 1B antibody
    • Nuclear Factor I B antibody
    • Nuclear factor I/B antibody
    • TGGCA Binding Protein antibody
    • TGGCA-binding protein antibody
    • TRANSCRIPTION FACTOR NFIB antibody
    see all

Anti-NFIB / NF1B2 antibody 画像



  • Predicted band size : 47 kDa


    NF1B2 detection by Western blot analysis. NF1B2 is detected in Rat Liver cells using NF1B2 rabbit polyclonal antibody at a 1/1000 dilution (lane 1). The signal is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
  • ab11989 staining of NFIB/NF1B2 in mouse cortex E15. PFA-fixed paraffin-embedded sections of mouse cortex E15 were incubated with ab11989 (1/1000) for 18 hours at 4°C. Antigen retrieval was performed by heat induction in sodium citrate buffer. Please see accompanying abreview for additional information.

    See Abreview

Anti-NFIB / NF1B2 antibody (ab11989) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Unfortunately, we find no problem with the lots we have sent.

We are sending new lots in order to help customer and assuming that the new lot will give them the results they are expecting.

Thanks!

The credit note ID for order PO-13779 is XXXXX.

The FOCR for order number PO-14643 is 1111586.

I hope this information will be helpful.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and surprised to hear that 2 vials of this antibody have been failed. We have sold many ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse cortex E15)
Specification Mouse cortex E15
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.025% TX100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Abcam user community

Verified customer

投稿 Jul 23 2008

Thank you for your e-mail. The source of the antibody told us " I am not sure if the 3 isoforms are expressed simultaneously in rat liver nuclear extract and I think that's not the case". We hope this information will clarify the reason for 1 band...

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The source of the antibody ab11989 has informed us " The antibody is raised against the peptide YVPSYDPSSPQTSQ. From our blast results, it seems the antibody should recognize all 3 isoforms". We hope this information will help you, please do not hes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"