Anti-NFATC4 (phospho S168 + S170) 抗体 (ab111929)
Key features and details
- Rabbit polyclonal to NFATC4 (phospho S168 + S170)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NFATC4 (phospho S168 + S170) antibody
NFATC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFATC4 (phospho S168 + S170) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human Breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab111929 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111929の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons. -
組織特異性
Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. -
配列類似性
Contains 1 IPT/TIG domain.
Contains 1 RHD (Rel-like) domain. -
ドメイン
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
翻訳後修飾
Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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参照データベース
- Entrez Gene: 4776 Human
- Entrez Gene: 73181 Mouse
- Omim: 602699 Human
- SwissProt: Q14934 Human
- SwissProt: Q8K120 Mouse
- Unigene: 77810 Human
- Unigene: 27908 Mouse
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別名
- cytoplasmic 4 antibody
- NF ATc4 antibody
- NF-AT3 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab111929 は論文での使用が確認できていません。