製品の概要

  • 製品名Anti-NFAT4 (phospho S165) antibody
    NFAT4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NFAT4 (phospho S165)
  • 特異性Detects endogenous levels of NFAT4 only when phosphorylated at serine 165.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphoryation site of serine 165 (SPSPPA)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21889978
WB 1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
  • 組織特異性Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
  • 配列類似性Contains 1 RHD (Rel-like) domain.
  • ドメインRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
  • 翻訳後修飾Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C80703 antibody
    • cytoplasmic 3 antibody
    • D8Ertd281e antibody
    • NF AT4 antibody
    • NF ATc3 antibody
    • NF-AT4 antibody
    • NF-ATc3 antibody
    • NFAC3_HUMAN antibody
    • NFATc3 antibody
    • NFATx antibody
    • Nuclear factor of activated T cells, cytoplasmic 3 antibody
    • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
    • Nuclear factor of activated T-cells antibody
    • T cell transcription factor NFAT4 antibody
    • T-cell transcription factor NFAT4 antibody
    see all

Anti-NFAT4 (phospho S165) antibody 画像

  • All lanes : Anti-NFAT4 (phospho S165) antibody (ab59204) at 1/500 dilution

    Lane 1 : HeLa cells treated with calcium (40nM, 30 mins)
    Lane 2 : HeLa cells treated with calcium (40nM, 30 mins) with immunizing phosphopeptide


    Predicted band size : 116 kDa
    Observed band size : 116 kDa

Anti-NFAT4 (phospho S165) antibody (ab59204) 使用論文

This product has been referenced in:
  • Saygili E  et al. Rate and irregularity of electrical activation during atrial fibrillation affect myocardial NGF expression via different signalling routes. Cell Signal : (2011). WB, ICC/IF ; Rat . Read more (PubMed: 21889978) »
  • Lin Z  et al. miR-23a functions downstream of NFATc3 to regulate cardiac hypertrophy. Proc Natl Acad Sci U S A 106:12103-8 (2009). WB ; Rat . Read more (PubMed: 19574461) »

See all 2 Publications for this product

Product Wall



The cell treatment protocol is as follows:

a. HeLa cells were plated on flasks or dishes containing growth medium (basic medium containing 10% serum).
b. When the cells were at the log phase and 80-90% confluent, the culture me...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"