Anti-NFAT1 抗体 [EPR2973] (ab92490)

製品の概要

  • 製品名Anti-NFAT1 antibody [EPR2973]
    NFAT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2973] to NFAT1
  • アプリケーション適用あり: WBmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human NFAT1 (UniProt Q13469).

  • ポジティブ・コントロール
    • Jurkat cell lysate, Ramos cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000 - 1/5000. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa).

    Is unsuitable for ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
    • 組織特異性Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
    • 配列類似性Contains 1 RHD (Rel-like) domain.
    • ドメインRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    • 翻訳後修飾In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
    • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI607462 antibody
      • cytoplasmic 2 antibody
      • KIAA0611 antibody
      • NF ATc2 antibody
      • NF ATp antibody
      • NF-ATc2 antibody
      • NF-ATp antibody
      • NFAC2_HUMAN antibody
      • NFAT 1 antibody
      • NFAT pre existing subunit antibody
      • NFAT pre-existing subunit antibody
      • NFAT transcription complex, preexisting component antibody
      • NFAT1 antibody
      • NFAT1-D antibody
      • NFATc2 antibody
      • NFATp antibody
      • Nuclear factor of activated T cells cytoplasmic 2 antibody
      • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
      • Nuclear factor of activated T cells pre-existing component antibody
      • Nuclear factor of activated T cells, preexisting component antibody
      • Nuclear factor of activated T-cells antibody
      • Preexisting nuclear factor of activated T cells 2 antibody
      • T cell transcription factor NFAT 1 antibody
      • T-cell transcription factor NFAT1 antibody
      see all

    Anti-NFAT1 antibody [EPR2973] 画像

    • All lanes : Anti-NFAT1 antibody [EPR2973] (ab92490) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 135 kDa (why is the actual band size different from the predicted?)

    Anti-NFAT1 antibody [EPR2973] (ab92490) 使用論文

    ab92490 has not yet been referenced specifically in any publications.

    Product Wall

    I am very pleased to hear you would like to accept our offer and test ab92490 in ChIP. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for ChIP. The code is inac...

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    Thank you very much for your interest in ab92490.

    To our knowledge, this product nor any of our other anti-NFAT products, have not been tested in ChIP. However by participating in our AbTrial program, you can now use our products in an unt...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"