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Recombinant human NF2/ Merlin with His tag purified from E.coli
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot number GR310755 and higher lots are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab88957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|Indirect ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Empty lane
Lane 3: NF2 / Merlin knockout HAP1 cell lysate (20 µg)
Lane 4: Empty lane
Lanes 1 - 4: Merged signal (red and green). Green - ab88957 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab88957 was shown to specifically react with NF2/Merlin in wild-type HAP1 cells. No band was observed when NF2 / Merlin knockout samples were examined. Wild-type and NF2/Merlin knockout samples were subjected to SDS-PAGE. Ab88957 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) ab216772 and IRDye® 680 Goat anti-Rabbit IgG (H + L) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.