Anti-NF-kB p65 (phospho S276) 抗体 (ab106129)

製品の概要

  • 製品名Anti-NF-kB p65 (phospho S276) antibody
    NF-kB p65 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NF-kB p65 (phospho S276)
  • 特異性ab106129 is specific for phosphorylated S276 NF-kB p65. It shows minimal reactivity by Western blot with non-phosphorylated NFkB p65, and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
  • アプリケーション適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human NF-kB p65 conjugated to Keyhole Limpet Haemocyanin (KLH). Immunogen = peptide corresponding to a region near phospho Serine 276 of the human protein

  • ポジティブ・コントロール
    • HeLa whole cell lysates; TNF inducible KBM5 cell lysates.
  • 特記事項

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 60 kDa.
ELISA 1/5000 - 1/25000.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
  • 配列類似性Contains 1 RHD (Rel-like) domain.
  • ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
    Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
    Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
    Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
  • 細胞内局在Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
  • Information by UniProt
  • 参照データベース
  • 別名
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    see all

Anti-NF-kB p65 (phospho S276) antibody 画像

  • All lanes : Anti-NF-kB p65 (phospho S276) antibody (ab106129) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : HeLa whole cell lysates, stimulated with TNF alpha


    Predicted band size : 60 kDa

Anti-NF-kB p65 (phospho S276) antibody (ab106129) 使用論文

This product has been referenced in:
  • Chen RY  et al. Effect of oridonin-mediated hallmark changes on inflammatory pathways in human pancreatic cancer (BxPC-3) cells. World J Gastroenterol 20:14895-903 (2014). WB ; Human . Read more (PubMed: 25356049) »
  • Takeuchi H  et al. The serine phosphatase SerB of Porphyromonas gingivalis suppresses IL-8 production by dephosphorylation of NF-?B RelA/p65. PLoS Pathog 9:e1003326 (2013). Read more (PubMed: 23637609) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Primary hippocampal neurons)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification Primary hippocampal neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Sep 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - Triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (UVB-induced murine skin tissue)
Specification UVB-induced murine skin tissue
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2013

Thank you for your reply.
Just to make sure there is no confusion, ab30623 & ab106129 are both NFkB phosphoS276 antibodies, not Re1A. Is this just an alternative name?
If you are looking for an antibody against NFkB phosphoS276, then I would re...

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Thank you for contacting Abcam.
As we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our you that all of our products are covered by our Abpromise, which guarantees th...

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DISCOUNT CODE: *****
Expiration date: *****

I am very pleased to hear you would like to accept our offer and test ab106129 in IHC and mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this off...

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Thank you for contacting us.

ab106129 has not been tested in IHC-P or mouse sections, therefore we do not know whether this product is suitable. I can offer a discount off a future purchase if you buy ab106129 now, test it in IHC and mouse a...

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Application Western blot
Sample Rat Cell lysate - whole cell (Spinal cord tissue)
Loading amount 20 µg
Specification Spinal cord tissue
Gel Running Conditions Reduced Denaturing (4-20% Tris-glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Kelly Atkins

Verified customer

投稿 Jun 18 2012

DISCOUNT CODE: ***
Expiration date: August 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab106129 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offe...

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Thank you for your inquiry.

I sincerely apologise for the inconvenience the long delay in availability of ab30623 is causing you.

We do offer an alternative antibody that is also specific for the phosphorylation site S276. Unfortuna...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"