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Synthetic peptide corresponding to Human NF-kB p65 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
NFkB is formed as a homo- or hetero-dimer. Subunits include p50 (NF-kB1), p65 (RelA), c-Rel, RelB and p52 (NF-kB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NF-kB have different functions in cells. Nuclear translocation of NF-kB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Our Abpromise guarantee covers the use of ab7552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|EMSA||Use a concentration of 0.5 - 1 µg/ml.|