製品の概要

  • 製品名
    Neutrophil Elastase Inhibitor Screening Kit
    Neutrophil Elastase キット 製品一覧
  • アッセイタイプ
    Quantitative
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Assay Buffer WM 1 x 25ml
    Inhibitor Control (3 mM, SPCK) Purple 1 x 100µl
    Neutrophil Elastase Green 1 vial
    Substrate Red 1 x 0.2ml
  • 研究分野
  • 関連性
    Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage.
  • 別名
    • Bone marrow serine protease
    • ELA2
    • ELANE
    • Elastase 2
    • Elastase 2 neutrophil
    • Elastase neutrophil expressed
    • GE
    • Granulocyte derived elastase
    • HLE
    • HNE
    • Human leukocyte elastase
    • Leukocyte elastase
    • Medullasin
    • NE
    • PMN E
    • PMN elastase
    • Polymorphonuclear elastase
    • SCN1
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab118971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (ab153768)

  • Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (ab114774)

  • Neutrophil Elastase Inhibition by SPCK
  • Neutrophil Elastase Activity Profile at Various Concentrations of SPCK

プロトコール

参考文献

This product has been referenced in:
  • Eggenschwiler R  et al. Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci Rep 6:38198 (2016). Functional Studies . Read more (PubMed: 27910942) »

See 1 Publication for this product

レビューと Q&A

RFU is an arbitrary unit and is dependent on the specific instrument and the gain/sensitivity, so unfortunately there is no way to give you a generic RFU to nmole correlation for calculation.

1. From what species is the elastase purified? Human.
2. What is the concentration of elastase provided? There is 15-22 mU of enzyme in the kit. The exact amount is proprietary.
3. What is the substrate? Eg. what is the sequence? MeOSuc - Ala -...

Read More

Yes. ab142178 is same as the substrate in ab118971.

Thank you for contacting us.

The substrate that is in the kit will be cleaved by active elastase, whether pure, as provided in the kit, or in biological samples. You could use the pure elastase in the kit as a standard for comparison to your...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録