製品の概要

  • 製品名
    Anti-Neutrophil Elastase antibody
    Neutrophil Elastase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neutrophil Elastase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, ICC, IHC-P, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein. Human leucocyte elastase.

  • ポジティブ・コントロール
    • Bone marrow.

アプリケーション

Our Abpromise guarantee covers the use of ab21595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20818377
ICC 1/50 - 1/100.
IHC-P 1/20 - 1/200. PubMed: 20837529We recommend antigen retrieval in a steamer or with 0.04% pepsin for 10 minutes at room temperature. See Abreview.
WB 1/200 - 1/500. Predicted molecular weight: 29 kDa.
IP 1/100 - 1/200.
ELISA 1/1000 - 1/2000.

ターゲット情報

  • 機能
    Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • 組織特異性
    Bone marrow cells.
  • 関連疾患
    Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • 配列類似性
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bone marrow serine protease antibody
    • ELA2 antibody
    • ELANE antibody
    • Elastase 2 antibody
    • Elastase 2 neutrophil antibody
    • Elastase neutrophil expressed antibody
    • Elastase-2 antibody
    • ELNE_HUMAN antibody
    • GE antibody
    • Granulocyte derived elastase antibody
    • HLE antibody
    • HNE antibody
    • Human leukocyte elastase antibody
    • Leukocyte elastase antibody
    • Medullasin antibody
    • NE antibody
    • Neutrophil elastase antibody
    • PMN E antibody
    • PMN elastase antibody
    • Polymorphonuclear elastase antibody
    • SCN1 antibody
    see all

画像

  • ab21595 staining Neutrophil Elastase in rat cornea tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    The section was deparaffinized with ethanol and the antigen was retrieved using a steamer. ab21595 was used at a 1/200 dilution, the secondary was an anti-rabbit IgG 1/5,000 also from Abcam. The DAB staining was performed using a DAB Peroxidase Substrate kit. The slides were counterstained with hematoxylin.
  • ab21595 staining neutrophil elastase (NE, green), DNA stained with propidium iodide (PI, red) in COPD sputum by ICC/IF (Immunocytochemistry/immunofluorescence). The sputum matrix contains condensed matrix traversed by thin neutrophil extracellular traps (NETs) strands associated with bacteria (arrowheads), non-activated polymorphonulear neutrophils (PMNs) (open arrow) and NETotic PMNs (solid arrow); asterisks indicate fully spread NETs.Cells were blocked with 10% normal goat seum in PBS containing 10 mM glycine and 0.2% Tween 20. Samples were incubated with primary antibody (1/50). ab96883 (1/100) was used as the secondary antibody.

  • ab21595 at 1/20 dilution staining rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The rat was challenged with LPS at 100ug/Kg i.p. The tissue sections were formaldehyde fixed and incubated with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG antibody was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Herrmann JB  et al. Complement C5a Receptor 1 Exacerbates the Pathophysiology ofN. meningitidisSepsis and Is a Potential Target for Disease Treatment. MBio 9:N/A (2018). IHC-P . Read more (PubMed: 29362231) »
  • Malcolm KC  et al. Neutrophil killing of Mycobacterium abscessus by intra- and extracellular mechanisms. PLoS One 13:e0196120 (2018). Read more (PubMed: 29672589) »

See all 44 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human ovarian cystedenocarcinoma tissue sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: bicarbonate buffer pH 6.0
Permeabilization
No
Specification
human ovarian cystedenocarcinoma tissue sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 03 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
Yes - tritonX-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse lung)
Specification
Mouse lung
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Nov 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization
No
Blocking step
BGB as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 31 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 10%
Username

Mr. John-Paul Jukes

Verified customer

投稿 Sep 26 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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