製品の概要

  • 製品名
    Anti-Neutrophil Elastase antibody
    Neutrophil Elastase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neutrophil Elastase
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, ICC, IHC-P, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein. Human leucocyte elastase.

  • ポジティブ・コントロール
    • Bone marrow.

アプリケーション

Our Abpromise guarantee covers the use of ab21595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20818377
ICC 1/50 - 1/100.
IHC-P 1/20 - 1/200. PubMed: 20837529We recommend antigen retrieval in a steamer or with 0.04% pepsin for 10 minutes at room temperature. See Abreview.
WB 1/200 - 1/500. Predicted molecular weight: 29 kDa.
IP 1/100 - 1/200.
ELISA 1/1000 - 1/2000.

ターゲット情報

  • 機能
    Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
  • 組織特異性
    Bone marrow cells.
  • 関連疾患
    Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
    Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
  • 配列類似性
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bone marrow serine protease antibody
    • ELA2 antibody
    • ELANE antibody
    • Elastase 2 antibody
    • Elastase 2 neutrophil antibody
    • Elastase neutrophil expressed antibody
    • Elastase-2 antibody
    • ELNE_HUMAN antibody
    • GE antibody
    • Granulocyte derived elastase antibody
    • HLE antibody
    • HNE antibody
    • Human leukocyte elastase antibody
    • Leukocyte elastase antibody
    • Medullasin antibody
    • NE antibody
    • Neutrophil elastase antibody
    • PMN E antibody
    • PMN elastase antibody
    • Polymorphonuclear elastase antibody
    • SCN1 antibody
    see all

画像

  • ab21595 staining Neutrophil Elastase in rat cornea tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    The section was deparaffinized with ethanol and the antigen was retrieved using a steamer. ab21595 was used at a 1/200 dilution, the secondary was an anti-rabbit IgG 1/5,000 also from Abcam. The DAB staining was performed using a DAB Peroxidase Substrate kit. The slides were counterstained with hematoxylin.
  • ab21595 staining neutrophil elastase (NE, green), DNA stained with propidium iodide (PI, red) in COPD sputum by ICC/IF (Immunocytochemistry/immunofluorescence). The sputum matrix contains condensed matrix traversed by thin neutrophil extracellular traps (NETs) strands associated with bacteria (arrowheads), non-activated polymorphonulear neutrophils (PMNs) (open arrow) and NETotic PMNs (solid arrow); asterisks indicate fully spread NETs.Cells were blocked with 10% normal goat seum in PBS containing 10 mM glycine and 0.2% Tween 20. Samples were incubated with primary antibody (1/50). ab96883 (1/100) was used as the secondary antibody.

  • ab21595 at 1/20 dilution staining rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The rat was challenged with LPS at 100ug/Kg i.p. The tissue sections were formaldehyde fixed and incubated with the antibody for 2 hours. A biotinylated goat anti-rabbit IgG antibody was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Maueröder C  et al. Capability of Neutrophils to Form NETs Is Not Directly Influenced by a CMA-Targeting Peptide. Front Immunol 8:16 (2017). IHC . Read more (PubMed: 28191006) »
  • Carmona-Rivera C  et al. A role for muscarinic receptors in neutrophil extracellular trap formation and levamisole-induced autoimmunity. JCI Insight 2:e89780 (2017). Read more (PubMed: 28194438) »

See all 39 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human ovarian cystedenocarcinoma tissue sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: bicarbonate buffer pH 6.0
Permeabilization
No
Specification
human ovarian cystedenocarcinoma tissue sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 03 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
Yes - tritonX-100
Fixative
Formaldehyde
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Verified customer

投稿 May 08 2014

According to the datasheet, this antibody is a non-purified whole serum product. That means, along with Ascites and cell culture supernatant products, the antibody concentration cannot be determined as there are too many other proteins in this solution...

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Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab21595. I would also appreciate if you can confirm some further details: Sometimes the antibody may react with your blocking solution to create this...

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The application IHC-Fr in mouse was added for two different reasons for these antibodies. ab21595 was validated for use in mouse in IHC-Fr following an abreview and the images were visualised using a secondary antibody conjugated to Alexa Fluor 594. ab...

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Vielen Dank für Ihre Email.

Ich habe diese wertvolle Information an unsere Datenblattabteilung weitergeleitet, und diese sollte das Datenblatt dann so schnell wie möglich aktualisieren und den Fehler beheben.

Nochmals Dank...

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Vielen Dank für Ihre Email.

Frau Zeeck ist in Ihren wohlverdienten Urlaub und deshalb muss ich Ihnen heute die schlechte Nachricht überbringen, dass wir keine anderen Antikörper haben, die bovine Neutrophile Elastase erkennen. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse lung)
Specification
Mouse lung
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Username

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Verified customer

投稿 Nov 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Prot K
Permeabilization
No
Blocking step
BGB as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 31 2012

Thank you for your enquiry regarding ab21595.

This antibody is not affinity purified but whole antiserum so the concentration if IgG is not determined. However, it is normally between a range of 10-15 mg/ml.

As the datasheet suggest...

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1-10 of 25 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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