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Our Abpromise guarantee covers the use of ab21595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20818377|
|ICC||1/50 - 1/100.|
|IHC-P||1/20 - 1/200. PubMed: 20837529We recommend antigen retrieval in a steamer or with 0.04% pepsin for 10 minutes at room temperature. See Abreview.|
|WB||1/200 - 1/500. Predicted molecular weight: 29 kDa.|
|IP||1/100 - 1/200.|
|ELISA||1/1000 - 1/2000.|
ab21595 staining neutrophil elastase (NE, green), DNA stained with propidium iodide (PI, red) in COPD sputum by ICC/IF (Immunocytochemistry/immunofluorescence). The sputum matrix contains condensed matrix traversed by thin neutrophil extracellular traps (NETs) strands associated with bacteria (arrowheads), non-activated polymorphonulear neutrophils (PMNs) (open arrow) and NETotic PMNs (solid arrow); asterisks indicate fully spread NETs.Cells were blocked with 10% normal goat seum in PBS containing 10 mM glycine and 0.2% Tween 20. Samples were incubated with primary antibody (1/50). ab96883 (1/100) was used as the secondary antibody.