製品の概要

  • 製品名Anti-Neutrophil antibody [NIMP-R14]
    Neutrophil 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [NIMP-R14] to Neutrophil
  • アプリケーション適用あり: ICC, Functional Studies, IHC-P, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils

  • エピトープThe monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
  • 特記事項

    The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS , 0.1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
  • ポリ/モノモノクローナル
  • クローン名NIMP-R14
  • ミエローマ210RCY3-Ag123
  • アイソタイプIgG2b
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1 mg, 6 hours before infection.
IHC-P Use at an assay dependent concentration.

This antibody has been reported to work well without antigen retrieval, or with retrieval in a 125 degree C decloaking chamber for 3 minutes with pH 6.5 buffer. HIER optimization may be required.

Flow Cyt Use at an assay dependent concentration.

5 x 105 cells were incubated with 10 µg/ml antibody.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. Tested with acetone-fixed tissue.

ターゲット情報

  • 細胞内局在Cell membrane; Lipid-anchor, GPI-anchor
  • 参照データベース
  • 別名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [NIMP-R14] 画像

  • ab2557 at 1/80 staining mouse blood cells (ip wash cells) by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a biotinylated rabbit anti-rat (mouse adsorbed) antibody.

    This image is courtesy of an Abreview submitted on 16 March 2006.

    See Abreview

  • ab2557 staining Neutrophil in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM sodium citrate. Samples were incubated with primary antibody (1/200 in 1x PBS/1% BSA/0.3% Triton X-100 buffer) for 1 hour at 25°C. A HRP-conjugated Goat anti-rat Ig polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab2557 staining Ly-6G/-6C in mouse spleen tissue by Immunohistochemistry (Frozen sections).
  • ab2557 staining Neutrophil in mouse tumor tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked, followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes. A biotin-conjugated rabbit anti-rat was used as secondary antibody at a 1/400 dilution.

Anti-Neutrophil antibody [NIMP-R14] (ab2557) 使用論文

This product has been referenced in:
  • Artinger K  et al. The Spleen Plays No Role in Nephrotoxic Serum Nephritis, but Constitutes a Place of Compensatory Haematopoiesis. PLoS One 10:e0135087 (2015). IF ; Mouse . Read more (PubMed: 26247770) »
  • Koh HS  et al. The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia. Nat Commun 6:6340 (2015). IHC-P ; Mouse . Read more (PubMed: 25790768) »

See all 47 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. VIctoria Thompson

Verified customer

投稿 Jun 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph6 citrate
Permeabilization No
Specification Skin
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. VIctoria Thompson

Verified customer

投稿 Jun 23 2016

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cells from bronchoaveolar lavage in balb/c mice)
Specification Cells from bronchoaveolar lavage in balb/c mice
Gating Strategy alive (DAPI-),CD45+, C11b+, CD11c- cells
Preparation Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

投稿 Feb 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
Sample Mouse Tissue sections (lung)
Specification lung
Permeabilization No
Fixative Formalin
Username

Abcam user community

Verified customer

投稿 Jan 02 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - Triton-X
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 05 2013

Thank you for contacting us.

Unfortunately, we cannot test every organ or tissue when we establish a product.However, if the knee joints were decalcified properly and the species is tested, I don't see any problems why these antibodies shoul...

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Thank you for contacting us.

We have tested this antibody with PFA fixed paraffin embedded tissue section and one of our customer have submitted positive Abreview for Immunohistochemistry on frozen sections using acetone fixative so it seems t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (blood)
Specification blood
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Trypsin
Permeabilization Yes - Triton X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Kate Schunke

Verified customer

投稿 Oct 02 2012

Thank you for your email.
I am sorry to hear that the replacement still gives you some background.

Could you please let me know if the conditions used were the same as with the first vial?
What antibody dilution was tried, and what ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"