Anti-Neutrophil 抗体 [7/4] (FITC) (ab53453)

製品の概要

  • 製品名Anti-Neutrophil antibody [7/4] (FITC)
    Neutrophil 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [7/4] to Neutrophil (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • アプリケーション適用あり: IHC-FoFr, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation: cultured bone marrow cells.

  • エピトープRecognizes the Ly-6B.2 antigen.
  • ポジティブ・コントロール
    • Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
  • 特記事項

    ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 一次抗体 備考ab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • ポリ/モノモノクローナル
  • クローン名7/4
  • ミエローマY3/Ag1.2.3
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 20038794
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use 10µl of the working dilution to label 106 cells in 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 細胞内局在Cell membrane; Lipid-anchor, GPI-anchor
  • 参照データベース
  • 別名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] (FITC) 画像

  • Immunohistochemical analysis of acetone-fixed frozen mouse spleen, labelling Neutrophil with ab53453 at a dilution of 1/100 incubated for 1 hour at 25°C in 5% goat serum in TBS. The secondary used (to amplify the signal) was a Goat anti-rat polyclonal Alexa Fluor® 488 conjugate at a 1/400 dilution.

    See Abreview

  • Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil:FITC (ab53453).

Anti-Neutrophil antibody [7/4] (FITC) (ab53453) 使用論文

This product has been referenced in:
  • Lamagna C  et al. B cell-specific loss of Lyn kinase leads to autoimmunity. J Immunol 192:919-28 (2014). Read more (PubMed: 24376269) »
  • Claudepierre MC  et al. Yeast virus-derived stimulator of the innate immune system augments the efficacy of virus vector-based immunotherapy. J Virol 88:5242-55 (2014). Flow Cyt . Read more (PubMed: 24574403) »

See all 9 Publications for this product

Product Wall

Swiss PO is used to denote "Swiss-Pathology Oxford," a specific strain at Oxford University.

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive neutrophils
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Verified customer

投稿 Nov 12 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive granulocytes
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投稿 Apr 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive monocytes
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投稿 Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen from immune mouse)
Permeabilization No
Specification Spleen from immune mouse
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Acetone
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投稿 Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy neutrophils
Username

Abcam user community

Verified customer

投稿 Dec 20 2011

Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope ...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"