製品の概要

  • 製品名Anti-Neutrophil antibody [7/4]
    Neutrophil 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [7/4] to Neutrophil
  • 特異性Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Neutrophil.

  • エピトープRecognizes the Ly-6B.2 antigen.
  • ポジティブ・コントロール
    • Flow cytometry: Mouse peripheral blood granulocytes.
  • 特記事項

    ab53457 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

     

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 一次抗体 備考ab53457 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • ポリ/モノモノクローナル
  • クローン名7/4
  • ミエローマY3/Ag1.2.3
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 細胞内局在Cell membrane; Lipid-anchor, GPI-anchor
  • 参照データベース
  • 別名
    • Ly-6B antibody
    • Ly6b antibody
    • Lymphocyte antigen 6B antibody
    • Lymphocyte antigen 6B Short name=Ly-6B antibody

Anti-Neutrophil antibody [7/4] 画像

  • Staining of mouse peripheral blood granulocytes with Rat anti mouse neutrophil (ab53457).

Anti-Neutrophil antibody [7/4] (ab53457) 使用論文

This product has been referenced in:
  • Skoczek DA  et al. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol 193:439-51 (2014). Read more (PubMed: 24907348) »
  • Huang L  et al. Interferon regulatory factor 7 protects against vascular smooth muscle cell proliferation and neointima formation. J Am Heart Assoc 3:e001309 (2014). IF ; Mouse . Read more (PubMed: 25304854) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us this afternoon and reporting the problems encountered with the most recent lot of the Anti-Neutrophil antibody [7/4] (ab53457). I am sorry to hear this antibody is not providing satisfactory results.

As discussed ...

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Thank you for contacting us.

This product has not been tested in that particular strain. However it is covered by our Abpromise and guaranteed to work on mouse in any of the tested applications listed on the datasheet.

I hope this...

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Thank you for contacting us.

Thank you for your inquiry. We have not tested MCA771GA on the Vm/Dk mouse strain. We ran a literature search, butwere unable to find any publications in which customers have tried this antibody on this mouse str...

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Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"