製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Neurturin
  • アプリケーション
    適用あり: IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    DVHSRYHTLQELSARE

    , corresponding to C terminal amino acids 176-191 of Mouse Neurturin

  • ポジティブ・コントロール
    • Mouse Brain and Rat Brain lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 22 kDa.
ELISA 1/64000.

ターゲット情報

  • 機能
    Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.
  • 関連疾患
    Defects in NRTN are a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
  • 配列類似性
    Belongs to the TGF-beta family. GDNF subfamily.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Neurturin antibody
    • Neurturin precursor antibody
    • NRTN antibody
    • NRTN_HUMAN antibody
    • NTN antibody
    • prepro-neurturin antibody
    see all

Anti-Neurturin antibody 画像

  • Anti-Neurturin antibody (ab63949) at 0.1 µg/ml (1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • ab63949 staining Neurturin in Mouse brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in 4% PFA, followed by 30% sucrose and permeabilized with Triton X-100 prior to blocking with 10% goat serum for 20 minutes at 23°C. The primary antibody was diluted 1/200 in the blocking buffer and incubated with the sample for 90 minutes at 23°C. A biotin-conjugated Rabbit anti-Goat was used as the secondary antibody, diluted 1/200.

Anti-Neurturin antibody (ab63949) 使用論文

ab63949 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
4% PF, followed by 30% sucrose
Permeabilization
Yes - Triton X 100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
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投稿 Nov 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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