製品の概要

  • 製品名
    Anti-Neurotrophin 3 antibody
    Neurotrophin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurotrophin 3
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Neurotrophin 3 aa 174-189 (internal sequence).
    Sequence:

    TVLGEIKTGNSPVKQY


    (Peptide available as ab182403)

  • ポジティブ・コントロール
    • Rat brain tissue sections and rat brain tissue lysate

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 0.18% Sodium chloride, 0.04% Dibasic monohydrogen sodium phosphate, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 23236374
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).Can be blocked with Neurotrophin 3 peptide (ab182403).
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

ターゲット情報

Anti-Neurotrophin 3 antibody 画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa


    Exposure time : 30 seconds

    ab9792 = Active human Neurotrophin 3 protein fragment

  • ab65804 at 0.5µg/ml staining Neurotrophin 3 in rat brain tissue sections.
  • All lanes : Anti-Neurotrophin 3 antibody (ab65804) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : HeLa whole cell lysate


    Predicted band size : 29 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • ABC immunocytochemistry of rat dorsal root ganglion neurons with ab65804. The DRG neurons were post-fixed with Zamboni's fixative for 20 minutes.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa

    ab9792 = Active human Neurotrophin 3 protein fragment

Anti-Neurotrophin 3 antibody (ab65804) 使用論文

This product has been referenced in:
  • Wang H  et al. Reduced neurotrophic factor level is the early event before the functional neuronal deficiency in high-fat diet induced obese mice. Metab Brain Dis N/A:N/A (2016). Read more (PubMed: 27624843) »
  • Wang J  et al. The Effect of Repeated Electroacupuncture Analgesia on Neurotrophic and Cytokine Factors in Neuropathic Pain Rats. Evid Based Complement Alternat Med 2016:8403064 (2016). WB . Read more (PubMed: 27800006) »

See all 10 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 20 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Mar 16 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Mar 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
10% buffered formalin
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投稿 Oct 20 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% in TGS)
Sample
Human Cell lysate - whole cell (Primary astrocytes)
Specification
Primary astrocytes
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

投稿 Jul 25 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jun 11 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jun 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Recombinant protein (HEK Cell supernatant)
Loading amount
10 µg
Specification
HEK Cell supernatant
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Mar 12 2013

Thank you for contacting us with your question. I have confirmed that the lab uses normal MCF7 and HeLa cell lysates to test ab65804. I hope this information is helpful, but please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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