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Synthetic peptide corresponding to Human Neurotrophin 3 aa 174-189 (internal sequence).
Our Abpromise guarantee covers the use of ab65804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23236374|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).Can be blocked with Neurotrophin 3 peptide (ab182403).|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.
Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
ABC immunocytochemistry of rat dorsal root ganglion neurons with ab65804. The DRG neurons were post-fixed with Zamboni's fixative for 20 minutes.
ab9792 = Active human Neurotrophin 3 protein fragment