Anti-Neurotrophin 3 抗体 (ab53685)

製品の概要

  • 製品名Anti-Neurotrophin 3 antibody
    Neurotrophin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurotrophin 3
  • 特異性ab53685 shows reactivity of less than 0.1% with mouse NGF, recombinant human BDNF or Neurotrophin 4.
  • アプリケーション適用あり: WB, ELISA, Inhibition Assay, IHC-Frmore details
  • 種交差性
    交差種: Rat, Chicken, Human
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids 139-149 of Mouse Neurotrophin 3 conjugated to BSA.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use a concentration of 1 - 10 µg/ml.
Inhibition Assay Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Neurotrophin 3 antibody 画像

  • All lanes : Anti-Neurotrophin 3 antibody (ab53685) at 1/500 dilution

    Lane 1 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 2 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 3 : Rat Brain (Age P21) Whole Tissue ysate
    Lane 4 : Rat Brain (Age P21) Whole Tissue ysate

    Secondary
    Dako Cytomation P0260 Conjugation: HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

Anti-Neurotrophin 3 antibody (ab53685) 使用論文

ab53685 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (day 11,5 Embryo)
Permeabilization No
Specification day 11,5 Embryo
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mr. Pierre-Yves Canto

Verified customer

投稿 Aug 29 2016

Thank you for your inquiry. This antibody to NT3 has been tested for biological activity using an in vitro assay based on the culture of embryonic sensory neurons from the chick dorsal root ganglia. The assay is performed according to the reference...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Brain Whole Tissue Lysate)
Loading amount 10 µg
Specification Brain Whole Tissue Lysate
Gel Running Conditions Reduced Denaturing (15% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Dec 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"