Anti-Neurotensin Receptor 1 抗体 (ab75555)

製品の概要

  • 製品名
    Anti-Neurotensin Receptor 1 antibody
    Neurotensin Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurotensin Receptor 1
  • 特異性
    ab75555 detects endogenous levels of total Neurotensin receptor 1 protein.
  • アプリケーション
    適用あり: ICC/IF, WB, Indirect ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized peptide derived from an internal sequence of human Neurotensin Receptor 1

  • ポジティブ・コントロール
    • Extracts from Jurkat cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
Indirect ELISA 1/5000.

ターゲット情報

  • 関連性
    Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.
  • 細胞内局在
    Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Plasma membrane. Integral membrane protein.
  • 参照データベース
  • 別名
    • High affinity levocabastine insensitive neurotensin receptor antibody
    • Neurotensin receptor 1 (high affinity) antibody
    • Neurotensin receptor type 1 antibody
    • NT R 1 antibody
    • NTR antibody
    • NTR1 antibody
    • NTR1_HUMAN antibody
    • NTRH antibody
    • NTRR antibody
    • NTSR 1 antibody
    see all

画像

  • All lanes : Anti-Neurotensin Receptor 1 antibody (ab75555) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ICC/IF image of ab75555 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab75555 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Oct 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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