All tags neuroscience 成熟ニューロン・マーカー

MAP2 antibody (ab5392)

成熟ニューロン・マーカー


神経系には役割の異なるさまざまな神経細胞、ニューロンが存在します。これらは神経性疾患における病態生理学的特徴といったマクロな視点から、ミクロな分子的特性まで、さまざまな観点から研究が進められています。

こうした研究において、さまざまな種類の成熟ニューロン(mature neuron)を識別することは非常に重要です。

ここでは成熟ニューロンの主なマーカー・タンパク質と、それらの発現細胞を識別するのに有用な抗体をご紹介します。


NeuN
An RNA binding protein that is highly specific for post-mitotic neurons, however, the following cell types do not express NeuN:

  • ​Golgi cells​
  • Purkinje cells
  • Olfactory bulb mitral cells
  • Retinal photoreceptor cells
  • Inferior olivary and dentate nucleus neurons
  • Sympathetic ganglion cells

Mouse cerebrum sections stained with anti-NeuN (green) (ab177487).

Browse all NeuN antibodies​​

Read about the advantages of our NeuN recombinant rabbit monoclonal antibody (ab177487)


MAP2

MAP2
A neuron-specific protein that promotes assembly and stability of the microtubule network.

Mouse primary neurons stained with anti-MAP2 (green) (ab5392).

​​​Browse all MAP2 antibodies​


160 kDa Neurofilament medium
Major intermediate filament found in neurons.

Rat cerebellum sections stained using ​anti-160 kDa neurofilament medium (ab64300).

Browse all 160 kDa neurofilament medium antibodies


200 kDa Neurofilament heavy
Major intermediate filament found in neurons.

Rat brain sections stained using anti-200 kDa neurofilament heavy (red) (ab8135).

Browse all 200 kDa neurofilament Heavy antibodies


Synaptophysin
Synaptic vesicle protein that regulates vesicle endocytosis in neurons.

Human iPS cell-derived neurons stained with anti-synaptophysin (green) (ab32127).

Browse all synaptophysin antibodies


PSD95
Synaptic protein that associates with receptors and the cytoskeleton.

Rat cerebellum sections stained with anti-PSD95 (ab18258).

Browse PSD95 antibodies


参考文献

  • El-Husseini, A. E. et al. PSD-95 involvement in maturation of excitatory synapses. Science 290, 1364–8 (2000).
    Kwon, S. E. & Chapman, E. R. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70, 847–54 (2011).
    Mullen, R. J., Buck, C. R. & Smith, A. M. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116, 201–211 (1992).
    Soltani, M. H. et al. Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma. Am. J. Pathol. 166, 1841–1850 (2005).
    Trojanowski, J. Q., Walkenstein, N. & Lee, V. M. Expression of neurofilament subunits in neurons of the central and peripheral nervous system: an immunohistochemical study with monoclonal antibodies. J. Neurosci. 6, 650–660 (1986).
    Yuan, a., Rao, M. V., Veeranna & Nixon, R. a. Neurofilaments at a glance. J. Cell Sci. 125, 3257–3263 (2012).
    El-Husseini, A. E. et al. PSD-95 involvement in maturation of excitatory synapses. Science 290, 1364–8 (2000).
  • Kwon, S. E. & Chapman, E. R. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70, 847–54 (2011).
  • Mullen, R. J., Buck, C. R. & Smith, A. M. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116, 201–211 (1992).
  • Soltani, M. H. et al. Microtubule-associated protein 2, a marker of neuronal differentiation, induces mitotic defects, inhibits growth of melanoma cells, and predicts metastatic potential of cutaneous melanoma. Am. J. Pathol. 166, 1841–1850 (2005).
  • Trojanowski, J. Q., Walkenstein, N. & Lee, V. M. Expression of neurofilament subunits in neurons of the central and peripheral nervous system: an immunohistochemical study with monoclonal antibodies. J. Neurosci. 6, 650–660 (1986).
  • Yuan, a., Rao, M. V., Veeranna & Nixon, R. a. Neurofilaments at a glance. J. Cell Sci. 125, 3257–3263 (2012).