製品の概要

  • 製品名Anti-Neuropilin 1 antibody
    Neuropilin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neuropilin 1
  • 特異性ab25998 is specific for Neuropilin 1. Immunogen sequence has low homology to Neuropilin 2.
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide, coupled to carrier protein, corresponding to amino acids within the N-terminal CUB domain of human Neuropilin 1. This sequence is highly conserved in rat and mouse Neuropilin 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/2000.
WB 1/1000. Predicted molecular weight: 102 kDa.

ターゲット情報

  • 機能The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
    The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
  • 組織特異性The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
  • 配列類似性Belongs to the neuropilin family.
    Contains 2 CUB domains.
    Contains 2 F5/8 type C domains.
    Contains 1 MAM domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A5 protein antibody
    • BDCA4 antibody
    • BLOOD DENDRITIC CELL ANTIGEN 4 antibody
    • CD304 antibody
    • Neuropilin-1 antibody
    • Neuropilin1 antibody
    • NP1 antibody
    • NPN1 antibody
    • NRP 1 antibody
    • NRP antibody
    • NRP1 antibody
    • NRP1_HUMAN antibody
    • transmembrane receptor antibody
    • Vascular endothelial cell growth factor 165 receptor antibody
    • VEGF165R antibody
    see all

Anti-Neuropilin 1 antibody 画像

  • All lanes : Anti-Neuropilin 1 antibody (ab25998) at 1/500 dilution

    Lane 1 : Untransfected COS-7 cells
    Lane 2 : COS-7 cells transfected with HA-tagged Neuropilin 1


    Predicted band size : 102 kDa
  • ICC/IF image of ab25998 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab25998 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25998, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Neuropilin 1 antibody (ab25998) 使用論文

This product has been referenced in:
  • Liu X  et al. Targeted delivery of Dicer-substrate siRNAs using a dual targeting peptide decorated dendrimer delivery system. Nanomedicine 10:1627-36 (2014). Read more (PubMed: 24965758) »
  • Joseph D  et al. Hormonal regulation and distinct functions of semaphorin-3B and semaphorin-3F in ovarian cancer. Mol Cancer Ther 9:499-509 (2010). WB ; Human . Read more (PubMed: 20124444) »

See all 4 Publications for this product

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Could you please contact Lucerna and tell them to contact us with the order number? Unfortunately the order number 200701952 you have provided is not helpful in locating your order. Lucerna will be knowing the orde...

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The concentration is 0.5 mg/ml. The antibody is titered to work at specific dilutions. This is why concentration is not mentioned on the datasheet. I have updated the datasheet with dilutions only to reflect this more clearly. For ICC/IF recommended d...

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We used a transfection model using an HA-tagged human Neuropilin 1 sequence. The expressed protein ran near 130 kDa, similar to the observed molecular weight found in other studies (Proc. Natl. Acad. Sci. U.S.A. 97:2573-2578(2000)).

Application Western blot
Sample Human Cell lysate - whole cell (HUVEC and HAEC)
Loading amount 25 µg
Specification HUVEC and HAEC
Treatment with or without VEGF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Oct 31 2007

The concentration of this antibody is titrated to the dilution specified on our datasheet, so we do not otherwise provide the concentration. If you have specific questions about the titer, then let us know.

Thank you for your enquiry. The concentration was not determined for this antibody as it was titrated to work at the following dilutions for these applications: ELISA 1/2000 and Western blot 1/500. Please contact us again if you have additional ques...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"