製品の概要

  • 製品名Anti-Neuropeptide Y antibody
    Neuropeptide Y 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Neuropeptide Y
  • 特異性The specificity of the NPY antiserum was determined by radioimmunoassay. Synthetic peptides, somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Arg8-vasopressin, vasoactive intestinal peptide, angiotensin II demonstrated less than 0.005% cross- reactivity and substance P less than 0.02%. Peptide YY and bovine pancreatic polypeptide demonstrated approx. 36% and 0.05% cross- reactivity respectively. Human peptide YY share 65% homology amino acid sequence for human NPY. Synthetic NPY completely abolished radio labelled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen.
  • アプリケーション適用あり: IHC-Fr, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig, Human
  • 免疫原

    Synthetic peptide:

    TPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY

    , corresponding to amino acids 30-64 of Human Neuropeptide Y. The peptide was coupled to Bovine Thyroglobulin.

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH.
  • 特記事項This antibody is excellent for immunohistochemistry. Good staining was achieved in rabbit brain and guinea pig gastrointestinal tract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-FoFr 1/500. PubMed: 19200078

ターゲット情報

Anti-Neuropeptide Y antibody 画像

  • ab6173 staining Neuropeptide Y in rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/10000) for 16 hours at 4°C. A Biotin-conjugated Donkey anti-sheep IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab6173 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6173 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Neuropeptide Y antibody (ab6173) 使用論文

This product has been referenced in:
  • Filipovic N  et al. Expression of PTHrP and PTH/PTHrP receptor 1 in the superior cervical ganglia of rats. Neuropeptides 48:353-9 (2014). Read more (PubMed: 25278090) »
  • Furlan A  et al. The transcription factor Hmx1 and growth factor receptor activities control sympathetic neurons diversification. EMBO J 32:1613-25 (2013). Read more (PubMed: 23591430) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: r.t°C
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Fabio Canneva

Verified customer

投稿 Jul 10 2014

We cannot guarantee this antibody works on paraffin embedded tissues. It has not been tested for this.

This antibody has not been tested for staining rat brain, so its reactivity with rat NPY is unknown, although certainly possible. You may be able to estimate the likelihood of cross-reactivity with this species by comparing the sequence of the syntheti...

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The affinity constant for this antibody has not been determined. The concentration of antibody in the sample has not been determined and the material is supplied as a whole serum. This specific antibody has been used for the past 15 or so years for man...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"