製品の概要

  • 製品名
    Anti-Neuropeptide Y antibody
    Neuropeptide Y 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neuropeptide Y
  • アプリケーション
    適用あり: IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Common marmoset
    交差が予測される動物種: Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.

    (Peptide available as ab32971.)

  • ポジティブ・コントロール
    • IHC-P: Human cerebral cortex tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/3000.

ターゲット情報

Anti-Neuropeptide Y antibody 画像

  • Immunohistochemistical detection of Neuropeptide Y using ab30914 on  formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain. Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins at room temperature. ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)

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  • Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None.  Blocking step: 1% BSA for 10 mins at 21°C. Primary antibody incubated at 1/1500 for 2 hours at 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.

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  • IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA;  tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.

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プロトコール

Anti-Neuropeptide Y antibody (ab30914) 使用論文

This product has been referenced in:
  • Bonini SA  et al. Cortical Structure Alterations and Social Behavior Impairment in p50-Deficient Mice. Cereb Cortex 26:2832-49 (2016). IHC . Read more (PubMed: 26946128) »
  • Fidzinski P  et al. KCNQ5 K(+) channels control hippocampal synaptic inhibition and fast network oscillations. Nat Commun 6:6254 (2015). Read more (PubMed: 25649132) »

See all 12 Publications for this product

Product Wall

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jun 22 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jun 22 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jun 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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投稿 Jun 20 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (nasal mucosa)
Permeabilization
No
Specification
nasal mucosa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

投稿 Jun 09 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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投稿 May 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Cortical Cells in culture)
Permeabilization
No
Specification
Rat Cortical Cells in culture
Fixative
PF 4%, Picrid Acid 0,2%
Username

Ms. Babben Tinner

Verified customer

投稿 Oct 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Duodenum (enteric nervous system))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Duodenum (enteric nervous system)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Formaldehyde
Username

Stephane Lezmi

Verified customer

投稿 May 25 2015

Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Recombinant protein (neuropeptide Y)
Specification
neuropeptide Y
Blocking step
PVP as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 24 2013

Unfortunately, we have not tested the cross reactivity of ab30914 against mouse PYY. With 69.4% homology with the immunogen we would not predict cross reactivity with mouse PYY.

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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