製品の概要

  • 製品名Anti-Neuropeptide Y antibody
    Neuropeptide Y 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neuropeptide Y
  • アプリケーション適用あり: IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Marmoset (common)
    交差が予測される動物種: Sheep, Rabbit, Chicken, Guinea pig, Cow, Pigeon, Pig, Zebrafish, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.

    (Peptide available as ab32971.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Cerebral Cortex FFPE section

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/3000.

ターゲット情報

Anti-Neuropeptide Y antibody 画像

  • ab30914 staining Neuropeptide Y in rat brain tissue section by IHC-FoFr (PFA perfusion fixed frozen sections). Rats were perfused with 4% PFA;  tissue was fixed overnight in the same fixative and were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody dilution 1/3000 (PBS + 0.3% Triton X100) at 20°C for 18 hours. The Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG dilution 1/1000 was used as secondary antibody. The image shows staining in the soma and processes of neurons with the expected distribution of Neuropeptide Y.

    See Abreview

  • Immunohistochemistical detection of Neuropeptide Y using ab30914 on  formaldehyde-fixed paraffin-embedded rat brain sections. The image shows NPY staining in the cerebellum and frontal cortex of the rat brain.  Formaldehyde antigen retrieval step: heat mediated in cittric acid ph6 buffer. Blocking step: 1% BSA for 10 mins @ rt°C. Ab30914 incubated at 1/1000 for 2 hours in TBS/BSA/azide. Secondary Antibody: anti-rabbit IgG conjugated to biotin (1/200)

    See Abreview

  • Immunohistochemistical detection of Neuropeptide Y antibody (ab30914) on formaldehyde fixed paraffin-embedded human foetal wall sectionsAntigen retrieval step: Heat mediated. Buffer: Citric acid pH6. Permeabilization: None.  Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/1500 for 2 hours @ 21°C in TBS/BSA/azide. Abcam's EXPOSE detection kit was used: ab80436. NB: In the submitted image arrowheads indicate positive features: red for ganglion cells in the Submucosa (Meissner's plexus), green for ganglion cells in the Muscularis externa (Auerbach's plexus). There is also a diffuse fine positivity throughout the smooth muscle cells of the externa.

    See Abreview

  • IHC image of Neuropeptide Y staining in human cerebral cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30914, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Neuropeptide Y antibody (ab30914) 使用論文

This product has been referenced in:
  • Raghanti MA  et al. Humans and great apes share increased neocortical neuropeptide Y innervation compared to other haplorhine primates. Front Hum Neurosci 8:101 (2014). IHC ; Human . Read more (PubMed: 24616688) »
  • Heinrich G  et al. Preserved energy balance in mice lacking FoxO1 in neurons of Nkx2.1 lineage reveals functional heterogeneity of FoxO1 signaling within the hypothalamus. Diabetes 63:1572-82 (2014). Mouse . Read more (PubMed: 24487022) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2016

Application Western blot
Sample Human Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kiney)
Permeabilization Yes - 0.1% troton x-100
Specification Kiney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 20 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (nasal mucosa)
Permeabilization No
Specification nasal mucosa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

投稿 Jun 09 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Triton x 100: 30 min
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Cortical Cells in culture)
Permeabilization No
Specification Rat Cortical Cells in culture
Fixative PF 4%, Picrid Acid 0,2%
Username

Ms. Babben Tinner

Verified customer

投稿 Oct 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Duodenum (enteric nervous system))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification Duodenum (enteric nervous system)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Formaldehyde
Username

Stephane Lezmi

Verified customer

投稿 May 25 2015

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Recombinant protein (neuropeptide Y)
Specification neuropeptide Y
Blocking step PVP as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 24 2013

Unfortunately, we have not tested the cross reactivity of ab30914 against mouse PYY. With 69.4% homology with the immunogen we would not predict cross reactivity with mouse PYY.

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"