Neurokinin A, Tachykinin peptide (ab120185)
Key features and details
- Tachykinin peptide
- CAS Number: 86933-74-6
- Soluble in water
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Neurokinin A, Tachykinin peptide -
製品の詳細
Tachykinin peptide -
別名
- NKA
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生理活性の詳細
Endogenous tachykinin peptide
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CAS 番号
86933-74-6 -
構造式
製品の特性
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分子量
1133.32 -
分子式
C50H80N14O14S -
配列
HKTDSFVGLM (Modifications: C-terminal amide) -
PubChem 登録番号
55582 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120185の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120185, Neurokinin A, Tachykinin peptide
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K562 cells were incubated at 37°C for 4h with vehicle control (0 μM) and 15 nM of neurokinin A (ab120185). Increased expression of p53 (ab26) correlates with an increase in neurokinin A concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab26 at 5 μg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120185 は 1 報の論文で使用されています。
- Drimousis S et al. Gender-Related Differences of Tachykinin NK2 Receptor Expression and Activity in Human Colonic Smooth Muscle. J Pharmacol Exp Ther 375:28-39 (2020). PubMed: 32764152