製品の概要

  • 製品名Anti-Neurogranin antibody
    Neurogranin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Neurogranin
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-KIKSGERGRKG

    , corresponding to internal sequence amino acids 45-55 of Human Neurogranin (NP_006167.1).

  • ポジティブ・コントロール
    • Human brain cerebral cortex lysate, Human brain hippocampus lysate, Mouse brain lysate and Rat brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.

ターゲット情報

  • 機能Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
  • 組織特異性In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
  • 配列類似性Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

Anti-Neurogranin antibody 画像

  • All lanes : Anti-Neurogranin antibody (ab99269) at 2 µg/ml

    Lane 1 : Human cerebellum lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 8 kDa
  • Anti-Neurogranin antibody (ab99269) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 8 kDa
    Primary incubation was 1 hour.
  • ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-Neurogranin antibody (ab99269) 使用論文

ab99269 has not yet been referenced specifically in any publications.

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