The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
組織特異性Restricted to brain (at protein level).
配列類似性Contains 1 EGF-like domain.
発生段階Expressed in brain of 3 months, 5 and 10-year-old individuals.
翻訳後修飾N-glycosylated. O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation. Phosphorylated; in intracellular and extracellular parts.
細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts.