リコンビナント
RabMAb

Anti-Neurogenin 2 抗体 [EPR4177] (ab109236)

製品の概要

  • 製品名
    Anti-Neurogenin 2 antibody [EPR4177]
    Neurogenin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4177] to Neurogenin 2
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Neurogenin 2 aa 100-200 (internal sequence).
    Database link: Q9H2A3
    (Peptide available as ab187850)

  • ポジティブ・コントロール
    • WB: SH-SY5Y and HepG2 cell lysates. ICC/IF: SH-SY-5Y cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
  • 追加情報
    Is unsuitable for ICC/IF or IP.
  • ターゲット情報

    画像

    • All lanes : Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified)

      Lane 1 : PC-12 cell lysate
      Lane 2 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 29 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 29 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Neurogenin 2 antibody [EPR4177] (ab109236) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    プロトコール

    参考文献

    ab109236 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    Yes - 0.3% triton
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 20 2014

    Thank you for contacting Abcam.

    I am sorry that ab109236 is not producing a strong 25 kDa band in Western blot for your mouse brain homogenate. As we discussed on the phone, I am sending you a positive control free of charge. I also recommend ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録