Rabbit polyclonal to Neurogenin 1 - N-terminal
Synthetic peptide from the N-terminal region of human Neurogenin 1 conjugated to KLH
A375 cell lysate. Human hepatocarcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71818 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Appears to mediate neuronal differentiation.
Expression restricted to the embryonic nervous system.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Western blot - Anti-Neurogenin 1 antibody - N-terminal (ab71818)
Anti-Neurogenin 1 antibody - N-terminal (ab71818) at 1/100 dilution + A375 cell lysate at 12.5 µg
Predicted band size:
Observed band size:
23 kDa (
why is the actual band size different from the predicted?
Additional bands at:
33 kDa, 45 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1 antibody - N-terminal (ab71818)
ab71818 at 1/50 dilution, staining Neurogenin 1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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