Anti-Neurofilament heavy polypeptide 抗体 [NF-01] (ab7795)

製品の概要

  • 製品名
    Anti-Neurofilament heavy polypeptide antibody [NF-01]
    Neurofilament heavy polypeptide 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide
  • 特異性
    This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.
  • アプリケーション
    適用あり: ELISA, IHC-Fr, ICC, IHC-P, WB, IHC - Wholemount, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
    交差が予測される動物種: a wide range of other species, Mammal
  • 免疫原

    A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 関連疾患
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody [NF-01] 画像

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.

  • ab7795 at a dilution of 1/1000, staining Neurofilament heavy polypeptide (Alexa 488 secondary at 1/2000) in rat brain tissue (30µm thick coronal sections) on free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  • Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488  (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

プロトコール

Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795) 使用論文

This product has been referenced in:
  • Song JL  et al. Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats. Exp Mol Med 49:e332 (2017). Read more (PubMed: 28524176) »
  • Clarke KE  et al. A robust and reproducible human pluripotent stem cell derived model of neurite outgrowth in a three-dimensional culture system and its application to study neurite inhibition. Neurochem Int 106:74-84 (2017). ICC/IF ; Human . Read more (PubMed: 28011165) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Recurrent Laryngeal Nerve)
Permeabilization
No
Specification
Recurrent Laryngeal Nerve
Fixative
Frozen in Isopentane and Liquid Nitrogen
Username

Stephen Cahalan

Verified customer

投稿 May 10 2017

Application
Immunocytochemistry
Sample
Mouse Cell (mESC)
Permeabilization
Yes - tritonx100
Specification
mESC
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

投稿 Nov 02 2016

Application
IHC - Wholemount
Sample
Rat Tissue (Dorsal root ganglion)
Specification
Dorsal root ganglion
Username

Dr. Shlomo Tsuriel

Verified customer

投稿 Feb 06 2014

Thank you for your inquiry.

I can confirm that we do not have ab73273 in our catalog anymore.

I can suggest ab7795 as alternative. It also recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various speci...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Differentiated embryonal carcinoma cells)
Specification
Differentiated embryonal carcinoma cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2009

Please refer the paper on the datasheet (Histochemistry 100, 495,1993) which features the use of this monoclonal antibody. "In similar preparations (the authors refer to pellet of brain proteins after cold depolymerization, described elsewhere in th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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