Anti-Neurofilament heavy polypeptide 抗体 (ab8135)

製品の概要

  • 製品名Anti-Neurofilament heavy polypeptide antibody
    Neurofilament heavy polypeptide 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurofilament heavy polypeptide
  • 特異性The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).
  • アプリケーション適用あり: IHC-FoFr, ICC/IF, ICC, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Cat, Human, Pig
  • 免疫原

    Recombinant fragment corresponding to Cow Neurofilament heavy polypeptide (C terminal).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 19075249
ICC/IF 1/1000.
ICC Use at an assay dependent concentration.
IHC-P 1/1000.
IHC-Fr 1/1000.
WB 1/10000.

ターゲット情報

  • 機能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 関連疾患Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性Belongs to the intermediate filament family.
  • 翻訳後修飾There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody 画像

  • ab8135 staining Neurofilament heavy polypeptide in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).
    Tissue was fixed with formaldehyde, blocked with 5% serum for 1 hour at 25°C and permeabilzed with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) for 12 hours at 25°C. An Alexa Fluor® 555 goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

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  • Immunocytochemical staining of 33B cells (rat oligodendroglioma) with ab8135 at 1/1000. Cells were cultured in DMEM/10% FCS on coverslips in 12 well plates. Cells were washed with PBS and fixed in 4% PFA until staining. Cells were washed teice for 2 minutes and then treated with 3% hydrogen peroxide in methanol for 15 minutes at room temperature. The sample was blocked in 10% goat serum in 1% PBSA and then incubated with the primary antibody in 1% PBSA for 2 hours at room temperature. A biotinylated goat anti-rabbit was used as the secondary at 1/200 and then incubated with ABC for 30 minutes at room temperature, followed by DAB in PBS (brown). The sample was counter-stained with haematoxylin.

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  • The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
  • ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.

    The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.

    The primary antibody was incubated for 1 hour at 25°C.

    Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.

    Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.

    Red = ab8135 staining Neurofilament heavy polypeptide

     

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Anti-Neurofilament heavy polypeptide antibody (ab8135) 使用論文

This product has been referenced in:
  • Vasconcelos DM  et al. Immune response and innervation signatures in aseptic hip implant loosening. J Transl Med 14:205 (2016). Read more (PubMed: 27387445) »
  • Gao Y  et al. Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Mol Med Rep 14:5481-5488 (2016). ICC/IF ; Rat . Read more (PubMed: 27840959) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% Normal buffered formalin
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投稿 Dec 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% Normal buffered formalin
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投稿 Dec 05 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (engineered nervous tissue)
Permeabilization No
Specification engineered nervous tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
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投稿 Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
Sample Human Tissue sections (fetal spinal cord)
Specification fetal spinal cord
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
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Mr. Heiko Locher

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投稿 Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Kelly Neuroblastoma Cell line)
Specification Kelly Neuroblastoma Cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Neuro2a)
Loading amount 6 µg
Specification Neuro2a
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Aug 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% TritonX-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (B50 Cell line)
Loading amount 6 µg
Specification B50 Cell line
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jul 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (33B)
Specification 33B
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Jul 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Neuro2a Cell Line)
Specification Neuro2a Cell Line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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投稿 Jul 06 2012

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