Anti-Neurofilament heavy polypeptide 抗体 (ab72996)

製品の概要

  • 製品名
    Anti-Neurofilament heavy polypeptide antibody
    Neurofilament heavy polypeptide 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Neurofilament heavy polypeptide
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.

  • ポジティブ・コントロール
    • Rat cerebral cortex lysate. Rat cortical neurons and glia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
ICC/IF 1/25000.

ターゲット情報

  • 機能
    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
  • 関連疾患
    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 翻訳後修飾
    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 200 kDa neurofilament protein antibody
    • CMT2CC antibody
    • Nefh antibody
    • Neurofilament heavy polypeptide 200kDa antibody
    • Neurofilament heavy polypeptide antibody
    • Neurofilament triplet H protein antibody
    • NF H antibody
    • NF-H antibody
    • NFH antibody
    • NFH_HUMAN antibody
    see all

Anti-Neurofilament heavy polypeptide antibody 画像

  • Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate

    Predicted band size : 115 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of rat cortical neurons and glia using ab72996 at 1/25000 dilution showing Neurofilament heavy polypeptide staining (red).

Anti-Neurofilament heavy polypeptide antibody (ab72996) 使用論文

This product has been referenced in:
  • Rotem N  et al. ALS Along the Axons - Expression of Coding and Noncoding RNA Differs in Axons of ALS models. Sci Rep 7:44500 (2017). IF . Read more (PubMed: 28300211) »
  • Bernal Sierra YA  et al. Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System. Front Mol Neurosci 10:70 (2017). IF . Read more (PubMed: 28360836) »

See all 9 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 7% · Temperature: 25°C
Sample
Mouse Cell (DRG neurons)
Specification
DRG neurons
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Mr. Shachar Dagan

Verified customer

投稿 Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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