Anti-Neurofilament heavy polypeptide 抗体 (ab72996)
Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neurofilament heavy polypeptide antibody
Neurofilament heavy polypeptide 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Neurofilament heavy polypeptide -
由来種
Chicken -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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精製度
IgY fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72996の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
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特記事項 |
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WB
1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa). |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
関連疾患
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 24587 Rat
- SwissProt: P16884 Rat
- Unigene: 108194 Rat
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別名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
画像
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Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate
Predicted band size: 115 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab72996 は 33 報の論文で使用されています。
- Guo Z et al. Engineering human skin model innervated with itch sensory neuron-like cells differentiated from induced pluripotent stem cells. Bioeng Transl Med 7:e10247 (2022). PubMed: 35111948
- Rose N et al. Receptor clustering and pathogenic complement activation in myasthenia gravis depend on synergy between antibodies with multiple subunit specificities. Acta Neuropathol 144:1005-1025 (2022). PubMed: 36074148
- Song DA et al. A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes. Mol Cell Proteomics 21:100418 (2022). PubMed: 36180036
- Iyer CC et al. Follistatin-induced muscle hypertrophy in aged mice improves neuromuscular junction innervation and function. Neurobiol Aging 104:32-41 (2021). PubMed: 33964607
- Otsu M et al. Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells. Front Cell Dev Biol 9:688283 (2021). PubMed: 34504841