製品の概要

  • 製品名Anti-Neurofibromin antibody
    Neurofibromin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurofibromin
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human, Zebrafish
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    The epitope recognized maps to a region between residue 2760 and the C terminus (residue 2818) of human Neurofibromin 1 using the numbering given in entry NP_000258.1 (GeneID 4763).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at a concentration of 2 - 10 µg/mg of lysate.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 319 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
    • 関連疾患Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
      Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
      Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
      Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
      Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
      Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 配列類似性Contains 1 CRAL-TRIO domain.
      Contains 1 Ras-GAP domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp686J1293 antibody
      • FLJ21220 antibody
      • Neurofibromatosis Noonan syndrome antibody
      • Neurofibromatosis related protein NF 1 antibody
      • Neurofibromatosis related protein NF1 antibody
      • neurofibromatosis type I antibody
      • Neurofibromatosis-related protein NF-1 antibody
      • Neurofibromin 1 antibody
      • Neurofibromin truncated antibody
      • Neurofibromin1 antibody
      • NF 1 antibody
      • NF antibody
      • NF1 antibody
      • NF1_HUMAN antibody
      • NFNS antibody
      • Type 1 Neurofibromatosis antibody
      • von Recklinghausen disease neurofibromin antibody
      • von Recklinghausen disease related protein VRNF antibody
      • VRNF antibody
      • WATS antibody
      • Watson disease related protein WSS antibody
      • Watson syndrome antibody
      • WSS antibody
      see all

    Anti-Neurofibromin antibody 画像



    • Predicted band size : 319 kDa


      Detection of Human Neurofibromin (NF1) By Western Blot and Immunoprecipitation. Samples: Whole cell lysate (40 ug - A; 200 ug - B) from mock treated (ctrl) or NF1 siRNA treated HeLa cells. Antibodies: Affinity purified rabbit anti Neurofibromin antibody (ab17963) used at 1 ug/ml for Western Blot (A) or 4 ug/200 ug lysate for IP (B). Detection: Chemiluminescence with an exposure times of 1 minute (A) or 10 seconds (B).

    Anti-Neurofibromin antibody (ab17963) 使用論文

    This product has been referenced in:
    • Shin J  et al. Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Dis Model Mech 5:881-94 (2012). WB ; Zebrafish . Read more (PubMed: 22773753) »
    • Hensley K  et al. Proteomic identification of binding partners for the brain metabolite lanthionine ketimine (LK) and documentation of LK effects on microglia and motoneuron cell cultures. J Neurosci 30:2979-88 (2010). WB . Read more (PubMed: 20181595) »

    See all 2 Publications for this product

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    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Zebrafish Tissue lysate - whole (whole 3 day post fertilization zebrafish embryos)
    Loading amount 50 µg
    Specification whole 3 day post fertilization zebrafish embryos
    Gel Running Conditions Reduced Denaturing (3-8% TA)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 09 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Lymphoblast cell line)
    Specification Lymphoblast cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
    Username

    Abcam user community

    Verified customer

    投稿 Jan 24 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"