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The epitope recognized maps to a region between residue 2760 and the C terminus (residue 2818) of human Neurofibromin 1 using the numbering given in entry NP_000258.1 (GeneID 4763).
Our Abpromise guarantee covers the use of ab17963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 319 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
Whole cell lysate (0.5-1.0mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: IP using rabbit anti-Neurofibromin antibody.
Lane 2: IP using ab17963 at 6 µg per reaction.
Lane 3: Control IgG.
For western blotting, ab17963 was used at 1 µg/ml.