製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Neurofascin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human Neurofascin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Mouse Spinal Cord.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 225 kDa (predicted molecular weight: 138 kDa).

ターゲット情報

画像

  • All lanes : Anti-Neurofascin antibody (ab90979) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate (ab7942)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Spinal Cord (Mouse) Tissue Lysate (ab50253)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 138 kDa
    Observed band size: 225 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 32 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    We hypothesize that the observed bands at 225 kDa correspond to the glycosylated form of Neurofascin. Abcam welcomes customer feedback.

プロトコール

参考文献

ab90979 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab90979.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録