製品の概要

  • 製品名Anti-Neurofascin antibody
    Neurofascin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neurofascin
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Mouse Neurofascin.

    (Peptide available as ab33377.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates; Brain (Mouse) Tissue Lysate - normal tissue Brain (Rat) Tissue Lysate - normal tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 155,186 kDa (predicted molecular weight: 138 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/1000.

ターゲット情報

Anti-Neurofascin antibody 画像

  • Anti-Neurofascin antibody (ab31457) at 1 µg/ml + Brain(Mouse)Whole Cell Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 138 kDa
    Observed band size : 155,186 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,55 kDa. We are unsure as to the identity of these extra bands.
    In Rat, Neurofascin has three observed isoforms , isoform 1 (138 kDa) which is known as Nfasc186, isoform 2 (133 kDa) and isoform 3 (132 kDa) which are both known as Nfasc155.  These proteins are highly glycosylated and their observed molecular weight in Western blot is 186 kDa and 155 kDa respectively.

    Mouse Neurofascin has a predicted molecular weight of 138 kDa (Swiss-Prot data) however the observed bands at 186 kDa and 155 kDa are thought to correspond to the glycosylated isoforms 1, 2 and 3 as observed in Rat (Sherman DL et al, 2005,PubMed:16337912) 
  • ICC/IF image of ab31457 stained human SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31475, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Ab31457 used on Rat cerebellum, Purkinje cells are variously positive: there is a clear punctate positivity of secondary dendritic processes, a strong membraneous positivity of the somata and also a single strongly positive fibre that seemingly enters the Granule Cell Layer indicated by red arrowheads axons of P.cells. The Glomeruli of the GCL are moderately positive. There is also a linear pattern of positivity within the GCL that may well be fibres.

    See Abreview

  • Immunohistochemistical detection of Neurofascin using antibody ab31457 on PFA perfusion-fixed rat brain sections. Primary antibody ab31457 was used at 1/1000 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. Some cytoplasmic staining was observed in granular neurons and neuronal processes (arrows on the figure on the right). The lefthand figure shows the hippocampal granular cell staining (objective X20), the righthand figure shows this at higher magnification. This staining is consistent with published observation of cytoplasmic localisation of Neurofascin in the cytoplasm of hippocampal cells and their axon initial segment (Burkarth

Anti-Neurofascin antibody (ab31457) 使用論文

This product has been referenced in:
  • Roche SL  et al. Loss of glial neurofascin155 delays developmental synapse elimination at the neuromuscular junction. J Neurosci 34:12904-18 (2014). WB, IHC-Fr, IHC - Wholemount ; Mouse . Read more (PubMed: 25232125) »
  • Chugh D  et al. Brain inflammation induces post-synaptic changes during early synapse formation in adult-born hippocampal neurons. Exp Neurol 250C:176-188 (2013). IHC-FoFr ; Mouse . Read more (PubMed: 24047952) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 05 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset Cerebellum)
Specification Marmoset Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid ph6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Mar 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"