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RabMAb

Anti-NeuroD2 抗体 [EPR5135] - ChIP Grade (ab109406)

製品の概要

  • 製品名
    Anti-NeuroD2 antibody [EPR5135] - ChIP Grade
    NeuroD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5135] to NeuroD2 - ChIP Grade
  • アプリケーション
    適用あり: ChIP, WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NeuroD2

  • ポジティブ・コントロール
    • Human cerebellum, Human hippocampus, and fetal brain lysates; Human brain tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    画像

    • All lanes : Anti-NeuroD2 antibody [EPR5135] - ChIP Grade (ab109406) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human hippocampus lysate
      Lane 3 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109406
    • ChIP analysis using ab109406 binding NeuroD2 in cerebral cortex tissue lysate from postnatal day 0 mouse. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (1/200) for 2 hours at 4°C.

      Positive control: Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.
      Primer sequence for PCR was:
      Nhlh2_F CTCACGAACTTCACCCGCAC
      Nhlh2_R AAATTGACTCCTCGGCCCTC
      Negative Control: ChIP performed with unrelated antibody (anti-GFP antibody).

      See Abreview

    参考文献

    This product has been referenced in:
    • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . Read more (PubMed: 26341353) »
    • Katsushima K  et al. Contribution of MicroRNA-1275 to Claudin11 Protein Suppression via a Polycomb-mediated Silencing Mechanism in Human Glioma Stem-like Cells. J Biol Chem 287:27396-406 (2012). WB ; Human . Read more (PubMed: 22736761) »

    See all 3 Publications for this product

    レビューと Q&A

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Mouse Cell lysate - whole cell (Neuro2A cell line)
    Specification
    Neuro2A cell line
    Treatment
    Transfected with NeuroD2-myc or emtpty vector pcDNA4
    Total protein in input
    200 µg
    Username

    Abcam user community

    Verified customer

    投稿 Jul 11 2014

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Mouse Cell lysate - whole cell (mouse N2A cell line)
    Specification
    mouse N2A cell line
    Treatment
    Transfected with NeuroD2 expressing vector
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 May 13 2014

    Application
    ChIP
    Detection step
    Other
    Sample
    Mouse Tissue lysate - whole (Cerebral cortex tissue from postnatal day 0 mouse)
    Specification
    Cerebral cortex tissue from postnatal day 0 mouse
    Negative control
    ChIP performed with unrelated antibody (anti-GFP antibody).
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Positive control
    Known target of NeuroD2, which is the promoter regions of Nhlh2 gene. Primer sequence for PCR was: Nhlh2_F CTCACGAACTTCACCCGCAC Nhlh2_R AAATTGACTCCTCGGCCCTC
    Username

    Abcam user community

    Verified customer

    投稿 Apr 18 2014

    According to our records, ab109406 was proving difficult to use and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the ...

    Read More

    Thank you for contacting us and for letting us know about the trouble with ab109406.

    I have attached the protocol that was used by the lab to test this antibody.

    Could you tell me a bit more about the problem and the protocol used, ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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