製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NeuroD2
  • アプリケーション
    適用あり: IHC-Fr, IP, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Gorilla, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse NeuroD2.

  • ポジティブ・コントロール
    • Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ; Brain (Human) Tissue Lysate - adult normal tissue; Cerebellum Mouse Tissue Lysate ; Mouse Hippocampus Tissue Lysate ; P0 Mouse Brain Mouse Tissue Lysate; P7 Mouse Brain Tissue Lysate ;

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/2000. Tissue was perfusion fixed in 10% formaldehyde. Staining appeared more intense without antigen retrieval.
IP Use at an assay dependent concentration.
IHC-P 1/2000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

Anti-NeuroD2 antibody 画像

  • All lanes : Anti-NeuroD2 antibody (ab104430) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 5 : P7 Mouse Brain Tissue Lysate
    Lane 6 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml
    Lane 7 : Cerebellum Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 8 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 9 : P0 Mouse Brain Mouse Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 10 : P7 Mouse Brain Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa,41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of NeuroD2 staining in a formalin fixed, paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104430, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC-P image of NeuroD2 staining on mouse CA1 hippocampus sections using ab104430 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours.

    See Abreview

  • IHC-P image of NeuroD2 staining on rat CA1 hippocampus sections using ab104430 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections wer then blocked using 1% BSA at 21°C for 10 min. The primary antibody was incubated at 21°C for 2 hours

    See Abreview

  • ab104430 staining NeuroD2 in 10% formaldehyde perfusion fixed 6 week old frozen mouse brain section (dentate gyrus). No antigen retrieval was performed. The section was incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104430, 1/2000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-NeuroD2 antibody (ab104430) 使用論文

This product has been referenced in:
  • Bayam E  et al. Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation. BMC Genomics 16:681 (2015). Mouse . Read more (PubMed: 26341353) »
  • Guner G  et al. NEUROD2 Regulates Stim1 Expression and Store-Operated Calcium Entry in Cortical Neurons. eNeuro 4:N/A (0). Read more (PubMed: 28303257) »

See all 2 Publications for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Cell lysate - whole cell (Neuro2A cell line)
Specification
Neuro2A cell line
Treatment
Transfected with NeuroD2-myc or empty vector pcDNA4
Total protein in input
100 µg
Username

Prof. Gulayse Ince Dunn

Verified customer

投稿 Jul 11 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録