製品の概要

  • 製品名Anti-NeuroD1 (phospho S274) antibody
    NeuroD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NeuroD1 (phospho S274)
  • 特異性ab78900 detects endogenous levels of NeuroD1 only when phosphorylated at serine 274.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human NeuroD1 around the phosphorylation site of serine 274 (P-L-SP-P-P).

  • ポジティブ・コントロール
    • Extracts from HeLa cells, treated with UV (15 min)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/10000.

ターゲット情報

  • 機能Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • 関連疾患Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 (phospho S274) antibody 画像

  • All lanes : Anti-NeuroD1 (phospho S274) antibody (ab78900) at 1/5000 dilution

    Lane 1 : Nuclear and cytoplasmic fractions from adult rat cerebellum
    Lane 2 : Nuclear and cytoplasmic fractions from adult rat cerebellum
    Lane 3 : Nuclear and cytoplasmic fractions from adult rat pancreas
    Lane 4 : Nuclear and cytoplasmic fractions from adult rat pancreas
    Lane 5 : Nuclear fractions from adult rat pineal gland pools at ZT14
    Lane 6 : Nuclear fractions from adult rat pineal gland pools at ZT14
    Lane 7 : Cytoplasmic fractions from adult pineal gland pools at ZT14
    Lane 8 : Cytoplasmic fractions from adult pineal gland pools at ZT14

    Lysates/proteins at 40 µg per lane.

    Secondary
    Biotin-conjugated goat anti-rabbit IgG polclonal at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Estela Munoz

    See Abreview

  • All lanes : Anti-NeuroD1 (phospho S274) antibody (ab78900) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, treated with UV (15 min) with no immunizing peptide
    Lane 2 : extracts from HeLa cells, treated with UV (15 min) with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa

Anti-NeuroD1 (phospho S274) antibody (ab78900) 使用論文

This product has been referenced in:
  • Castro AE  et al. Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland. J Pineal Res 58:439-51 (2015). WB ; Rat . Read more (PubMed: 25752781) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (Pineal Gland)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Pineal Gland
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Estela Munoz

Verified customer

投稿 Jun 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"