製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to NeuroD1
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, WB, ELISA, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human, Apteronotus leptorhynchus
    交差が予測される動物種: Rat, Sheep, Cow
  • 免疫原

    Recombinant fragment with tag: QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT , corresponding to amino acids 201-300 of Human NeuroD1

  • ポジティブ・コントロール
    • IMR-32 (human neuroblastoma) whole cell lysate and human ovary, clear cell carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA Use at an assay dependent concentration. The detection limit for ab60704 is approximately 0.03ng/ml as a capture antibody.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • 関連疾患
    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾
    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 antibody 画像

  • Anti-NeuroD1 antibody (ab60704) + IMR-32 whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of formaldehyde fixed human cerebellum sections incubated with ab60704 for 20 minutes at 25°C in a concentration of 1/400. The blocking step was performed with 3% H2O2 for 10 minutes at 25ºC. The secondary antibody used was a polyclonal goat anti-mouse/rabbit HRP conjugate, used undiluted.

    See Abreview

  • ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
  • Immunofluorescence image of NeuroD1 (ab60704) on D3 mouse embryonic stem cells after 14 days of differentiation. The cells were fixed in paraformaldehyde and permeabilized in block solution. In this image you can see NeuroD1 positive staining with ab60704 in green.

    See Abreview

  • ab60704 staining NeuroD1 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton in TBS for 30 minutes and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500) for 15 hours at 4°C. An undiluted Alexa Fluor®633-conjugated Goat anti-mouse monoclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SHSY-5Y cells stained with ab60704 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60704, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NeuroD1 antibody (ab60704) 使用論文

This product has been referenced in:
  • Wakabayashi T  et al. Diabetes Impairs Wnt3 Protein-induced Neurogenesis in Olfactory Bulbs via Glutamate Transporter 1 Inhibition. J Biol Chem 291:15196-211 (2016). Read more (PubMed: 27226528) »
  • Kowno M  et al. Prohibitin 2 regulates the proliferation and lineage-specific differentiation of mouse embryonic stem cells in mitochondria. PLoS One 9:e81552 (2014). Mouse . Read more (PubMed: 24709813) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Foetal pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE buffer pH9.0
Permeabilization
Yes - TritonX100 (0.5%) in PBS
Specification
Foetal pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Username

Colin Plumb

Verified customer

投稿 Feb 03 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - TritonX-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

投稿 Nov 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cortical neurons)
Specification
cortical neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 07 2016

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization
No
Specification
brain
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
Permeabilization
No
Specification
cerebellum
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Sample
Pig Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 15 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 30min 0.5 Triton TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Ellie Tuck

Verified customer

投稿 Nov 18 2010

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録