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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-NeuroD1 antibody
NeuroD1 抗体 (10件) 一覧
Description
Rabbit polyclonal to NeuroD1
Specificity
ab16508 is raised against an immunogen specific for human, rat and mouse NeuroD1. The immunizing peptide also has 100% identity (based on a BLAST search) with mouse and rat NeuroD2, human NeuroD4, mouse and human NeuroD6 and rat MIST1.
Tested applications
IHC-Fr, IHC-FoFr, ICC/IF, ICC, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NeuroD1.
Positive control
mouse lung, human brain, fixed cultures of murine neurones differentiated for 3 days from murine neocortical precursor cells (E14).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20531234)
ICC/IF: Use a concentration of 1 - 5 µg/ml.
ICC: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 38-43 kDa (predicted molecular weight: 38-43 kDa).
Target
Function
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
Involvement in disease
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ13562
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
Database links
- Entrez Gene: 4760 Human
- Entrez Gene: 18012 Mouse
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q60867 Mouse
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
see all
Anti-NeuroD1 antibody images:
Immunocytochemistry - NeuroD1 antibody (ab16508)
[A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging). Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.
[B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody.
[C] blue = TO-PRO-3 (nuclear) staining
[D] Overlay of A, B and C demonstrates localisation of NeuroD1 to the cell bodies of these immature neurons.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Western blot - NeuroD1 antibody (ab16508)
Lane 1 : Marker
Lanes 2 - 3 : Anti-NeuroD1 antibody (ab16508) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Human) Tissue Lysate (ab7925) at 20 µg
Lane 3 : Brain (Human) Tissue Lysate (ab7925) at 20 µg with NeuroD1 peptide (ab17100) at 1 µg/ml
Secondary
Lanes 2 - 3 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 38-43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~49 kDa,~60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-NeuroD1 antibody (ab16508)
This product has been referenced in:
- Klisch TJet al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF; Mouse.Read more (PubMed: 21300888) »
- Puligilla Cet al. Sox2 induces neuronal formation in the developing mammalian cochlea. J Neurosci 30:714-22 (2010). IHC-P; Mouse.Read more (PubMed: 20071536) »
See all 6 publications for this product
Publishing research using ab16508? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"