製品の概要

  • 製品名Anti-NeuroD1 antibody [3H8]
    NeuroD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3H8] to NeuroD1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant Human NeuroD1 protein.

  • ポジティブ・コントロール
    • WB: transfected 293T cell line IHC-P: Human testis tissue ICC/IF: HeLa cell

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab117562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. (recombinant protein).

ターゲット情報

  • 機能Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
  • 関連疾患Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

Anti-NeuroD1 antibody [3H8] 画像

  • ab117562, at 5 µg/ml, staining NeuroD1 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-NeuroD1 antibody [3H8] (ab117562)

    Lane 1 : transfected 293T cell line lysate
    Lane 2 : non-transfected 293T cell line lysate


    Predicted band size : 40 kDa
  • ab117562, at 10 µg/ml, staining NeuroD1 in Hela cell by Immunofluorescence.
  • Detection limit for recombinant proprietary tagged NeuroD1 is approximately 0.03 ng/ml as a capture antibody.

Anti-NeuroD1 antibody [3H8] (ab117562) 使用論文

ab117562 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Testis
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"