製品の概要

  • 製品名
    Anti-Neurocan antibody [650.24]
    Neurocan 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [650.24] to Neurocan
  • 特異性
    The clone number has been updated from (2Q174) to (650.24) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: ICC, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Full length native core protein (purified) (Rat)

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab125021 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    650.24
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab26003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/5.
IHC-P 1/1000.
WB Use a concentration of 1 - 2 µg/ml.
IP Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能
    May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
  • 組織特異性
    Brain.
  • 配列類似性
    Belongs to the aggrecan/versican proteoglycan family.
    Contains 1 C-type lectin domain.
    Contains 2 EGF-like domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
    Contains 1 Sushi (CCP/SCR) domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Chondroitin sulfate proteoglycan 3 antibody
    • Cspg3 antibody
    • Ncan antibody
    • NCAN_HUMAN antibody
    • NEUR antibody
    • Neurocan core protein antibody
    • Neurocan proteoglycan antibody
    see all

参考文献

This product has been referenced in:
  • Noguchi A  et al. Juvenile Hippocampal CA2 Region Expresses Aggrecan. Front Neuroanat 11:41 (2017). Read more (PubMed: 28539874) »
  • Haenold R  et al. NF-?B controls axonal regeneration and degeneration through cell-specific balance of RelA and p50 in the adult CNS. J Cell Sci 127:3052-65 (2014). Read more (PubMed: 24860143) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
chABC overnight
Specification
hippocampus
Blocking step
licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
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投稿 Oct 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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