製品の概要

  • 製品名
    Anti-Neurobeachin antibody
    Neurobeachin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Neurobeachin
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog, Pufferfish, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-DFNRWHYEHQNRY

    , corresponding to C terminal amino acids 2934-2946 of Human Neurobeachin

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    This antibody gave a positive result in Peptide ELISA against the immunizing peptide. Antibody detection limit dilution 1/8000.


    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 327 kDa). Approx 300 kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins.
    • 組織特異性
      Predominant in many brain structures. Also expressed at medium levels in spleen, thymus, prostate, testis and ovary. Low level expression is seen in heart, kidney, pancreas, skeletal muscle and intestine.
    • 配列類似性
      Belongs to the WD repeat neurobeachin family.
      Contains 1 BEACH domain.
      Contains 5 WD repeats.
    • ドメイン
      RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
    • 細胞内局在
      Cytoplasm. Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BCL8B antibody
      • FLJ10197 antibody
      • KIAA1544 antibody
      • Lysosomal trafficking regulator 2 antibody
      • Lysosomal-trafficking regulator 2 antibody
      • LYST2 antibody
      • NBEA antibody
      • NBEA_HUMAN antibody
      • Neurobeachin antibody
      • Protein BCL8B antibody
      • Protein neurobeachin antibody
      • RP11-270C18.1 antibody
      see all

    画像

    • All lanes : Anti-Neurobeachin antibody (ab58563) at 1 µg/ml

      Lane 1 : Lysate prepared from non transfected HEK293 T cells
      Lane 2 : Lysate prepared from HEK293 T cells transfected with mouse Neurobeachin

      Developed using the ECL technique

      Predicted band size : 327 kDa
      Observed band size : 300 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab58563 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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