Anti-NeuN 抗体 - Neuronal Marker (ab104225)

製品の概要

  • 製品名Anti-NeuN antibody - Neuronal Marker
    NeuN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NeuN - Neuronal Marker
  • アプリケーション適用あり: WB, IHC-FrFl, IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human, Pig
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Recombinant fragment corresponding to Human NeuN aa 1-100 (N terminal).
    Sequence:

    MAQPYPPAQYPPPPQNGIPAEYAPPPPHPTQDYSGQTPVPTEHGMTLYTP AQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQPKR

  • ポジティブ・コントロール
    • Rat brain cortex tissue This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: SKNSH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-FrFl Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500 - 1/1000.
ICC/IF 1/500 - 1/1000.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

Anti-NeuN antibody - Neuronal Marker 画像

  • IHC image of NeuN staining in rat cerebellum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104225, 1in500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

  • IHC image of NeuN staining in human cerebellum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104225, 1in500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of NeuN staining in mouse cerebellum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104225, 1in500 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemical analysis of paraformaldehyde fixed frozen section Rat hippocampus tissue labeling NeuN with ab104225 at 1/1000 dilution.

  • ab104225 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104225 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • IHC-P image of FOX3/NeuN staining using rat DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).

    See Abreview

  • ab104225 at 1/1000 dilution, staining FOX3 (red) in rat brain cortex and counterstained for DNA in blue.

  • IHC-P image of FOX3/NeuN staining using mouse DRG using ab104225 (1:500). The sections were subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were blocked using 1% BSA for 10 mins at 21°C. ab104225 was incubated for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to Rabbit IgG conjugated to biotin (1:200).

    See Abreview



  • Predicted band size : 48 kDa

    Western blot of rat brain homogenates staining ab104225 at dilution 1/10000

Anti-NeuN antibody - Neuronal Marker (ab104225) 使用論文

This product has been referenced in:
  • Kaufling J & Aston-Jones G Persistent Adaptations in Afferents to Ventral Tegmental Dopamine Neurons after Opiate Withdrawal. J Neurosci 35:10290-303 (2015). IHC ; Rat . Read more (PubMed: 26180204) »
  • Murlidharan G  et al. Unique glycan signatures regulate adeno-associated virus tropism in the developing brain. J Virol 89:3976-87 (2015). ICC/IF ; Mouse . Read more (PubMed: 25631075) »

See all 17 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.1% triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 06 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton-X
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (DRG)
Specification DRG
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (DRG)
Specification DRG
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 29 2012

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numero...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.


Ich habe die Homologie der Immunogene mit Callithrix jacchus verglichen:

ab53025 : 100%
ab104225 : 96%


Unseres Wissens nach...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (Embryonic day 14.5))
Specification Brain (Embryonic day 14.5)
Fixative Paraformaldehyde
Permeabilization Yes - PBS with 0.5% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization Yes - 0.1% Triton-X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization Yes - 0.1% Triton-X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2012

Thank you for your email. I am very sorry that both antibodies did not work as expected. As per your request, I have aranged for a refund for the antibodies:

Your credit note ID is xxx.

I am sorry that these 2 antibodies did not p...

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