This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Hippocampus; Forebrain; Cortex.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 73 kDa).
Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitement of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma.
Netrin G2 Ligand contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Please note that U87MG was used as a negative control.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116697 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.