Anti-NET1 抗体 (ab5914)
Key features and details
- Goat polyclonal to NET1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NET1 antibody
NET1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NET1 -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human NET1 aa 500 to the C-terminus (C terminal). (NP_001040625.1)
Database link: NP_005854.2 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10276 Human
- SwissProt: Q7Z628 Human
- Unigene: 25155 Human
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別名
- ARHG8_HUMAN antibody
- ARHGEF8 antibody
- GLYT2 antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (5)
ab5914 は 5 報の論文で使用されています。
- Sun X et al. Inhibition of NET-1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Exp Ther Med 17:2334-2340 (2019). PubMed: 30867719
- Levi L et al. Bacterial genotoxins promote inside-out integrin ß1 activation, formation of focal adhesion complexes and cell spreading. PLoS One 10:e0124119 (2015). WB . PubMed: 25874996
- Srougi MC & Burridge K The Nuclear Guanine Nucleotide Exchange Factors Ect2 and Net1 Regulate RhoB-Mediated Cell Death after DNA Damage. PLoS One 6:e17108 (2011). WB ; Human . PubMed: 21373644
- Dutertre M et al. Estrogen regulation and physiopathologic significance of alternative promoters in breast cancer. Cancer Res 70:3760-70 (2010). WB ; Human . PubMed: 20406972
- García-Mata R et al. The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. Mol Cell Biol 27:8683-97 (2007). PubMed: 17938206