製品の概要

  • 製品名
    Anti-Nestin antibody [Rat-401]
    Nestin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Rat-401] to Nestin
  • アプリケーション
    適用あり: ICC/IF, ICC, IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
    非交差種: Human
  • 免疫原

    Nestin purified from embryonic rat spinal cord.

  • ポジティブ・コントロール
    • WB: MEF1 whole cell lysate. ICC/IF: PC12 cells. IHC-P: 6 week old rat brain FFPE tissue sections.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 170, 350 kDa (predicted molecular weight: 177 kDa). The use of BSA as blocking agent is not recommended.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • 組織特異性
    CNS stem cells.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • 翻訳後修飾
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

Anti-Nestin antibody [Rat-401] 画像

  • Immunocytochemical analysis of Rat neural stem cells from the hippocampus, labelling Nestin with ab11306. Samples were fixed in paraformaldehyde, permeablized in triton x-100 and blocked in 1% BSA for 40 minutes at room temperature. Incubation with ab11306 diluted 1/133 for 12 hours at 4°C. Secondary antibody was a polyclonal goat anti mouse FITC diluted 1/200.

    See Abreview

  • Anti-Nestin antibody [Rat-401] (ab11306) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 177 kDa


    Exposure time : 20 minutes
  • ab11306 staining Nestin in rat lateral ventricle (subventricular zone)  sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with  1% BSA for 10 minutes at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody 1/200 (TBS/BSA/azide pH7.5) for 24 hours. A Biotin-conjugated Goat polycolonal to mouse IgG, dilution 1/200, was used as secondary antibody. The image shows clear staining in cells of the rat subventricular zone of the lateral ventricle.

    See Abreview

  • ICC/IF image of ab11306 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11306, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.
  • IHC image of Nestin staining in 6 week old rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11306, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Nestin antibody [Rat-401] (ab11306) 使用論文

This product has been referenced in:
  • Fang L  et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). WB ; Human . Read more (PubMed: 27246838) »
  • Lai Q  et al. NMDA receptors promote neurogenesis in the neonatal rat subventricular zone following hypoxic-ischemic injury. Mol Med Rep 13:206-12 (2016). Rat . Read more (PubMed: 26548659) »

See all 27 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - Brij
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 21 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 10 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jun 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (neural stem cell,hippocampus)
Specification
neural stem cell,hippocampus
Fixative
Paraformaldehyde
Permeabilization
Yes - triton x-100
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. san san

Verified customer

投稿 Aug 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult rat svz of lateral ventricle)
Specification
adult rat svz of lateral ventricle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6. Not a buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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