Anti-Nestin 抗体 - Neural Stem Cell Marker (ab7659)

製品の概要

  • 製品名
    Anti-Nestin antibody - Neural Stem Cell Marker
    Nestin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • 特異性
    Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Dog, Human
    交差が予測される動物種: Rat, Rabbit, Hamster
  • 免疫原

    Synthetic peptide:

    ALETESQDSAEPSGSEE

    , corresponding to amino acids 1481-1497 of Human Nestin.

  • 特記事項
    ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 組織特異性
      CNS stem cells.
    • 配列類似性
      Belongs to the intermediate filament family.
    • 発生段階
      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻訳後修飾
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    画像

    • Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg

      Secondary
      IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml

      Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
      Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG HRP at 1/20000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 176 kDa
      Observed band size : 230 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

      A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.

    プロトコール

    参考文献

    This product has been referenced in:
    • Drew KL  et al. Arctic ground squirrel neuronal progenitor cells resist oxygen and glucose deprivation-induced death. World J Biol Chem 7:168-77 (2016). Read more (PubMed: 26981205) »
    • Tingling JD  et al. CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models. PLoS One 8:e69560 (2013). IF . Read more (PubMed: 23894503) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (neural precourser cell derived from iPSC)
    Specification
    neural precourser cell derived from iPSC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Apr 13 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Sep 11 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (Olfactory Stem Cell)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cell
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Sep 08 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Olfactory Stem cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Jul 16 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Olfactory stem cells)
    Permeabilization
    Yes - 0,1% Triton 100X
    Specification
    Olfactory stem cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Jun 22 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Jun 22 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Microcebus (Mouse Lemur) Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Antoine Veron

    Verified customer

    投稿 Jun 22 2015

    Thank you for contacting Abcam regarding ab7659. These applications have been listed as unsuitable as we have obtained a range of feedback stating that the antibody is staining nonspecifically in these applications.  If you have not used this a...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    Yes - Methanol
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 18 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neural precursor cells/neurospheres)
    Loading amount
    10 µg
    Specification
    neural precursor cells/neurospheres
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    投稿 May 18 2006

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録