製品の概要

  • 製品名Anti-Nestin antibody
    Nestin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nestin
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 325 and 375 of human Nestin (NP_006608.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 5 - 10 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 177 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 組織特異性CNS stem cells.
    • 配列類似性Belongs to the intermediate filament family.
    • 発生段階Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻訳後修飾Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody 画像

    • All lanes : Anti-Nestin antibody (ab85684) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 177 kDa


      Exposure time : 3 minutes
    • Nestin was immunoprecipitated using ab85684 at 10 µg/mg of whole cell lysate from HeLa cells.
      20% of the IP was loaded, and probed with ab85684 at 0.4 µg/ml.
      Detection: chemiluminescence with exposure time of 30 seconds.

    Anti-Nestin antibody (ab85684) 使用論文

    ab85684 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab85684.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"